相关实验视频

Updated: Jul 28, 2025

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

通过单细胞转录学揭示的体感神经元类型及其神经网络

Kaikai Wang¹, Bing Cai¹, Yuran Song¹

¹Guangdong Institute of Intelligence Science and Technology, Hengqin 519031, Zhuhai, Guangdong, China; Research Unit of Pain Medicine, Chinese Academy of Medical Sciences, Hengqin, Zhuhai, China.

Trends in neurosciences

|June 2, 2023

概括

单细胞RNA测序 (scRNA-seq) 揭示了背部根 (DRG) 神经元类型. 本综述整合了scRNA-seq的发现,以标准化DRG神经元分类,以便更好地了解体感系统.

科学领域:

神经科学是一个神经科学.
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

单细胞RNA测序 (scRNA-seq) 能够对背部根性质 (DRG) 神经元进行详细的分析.
之前的研究中各种分类标准阻碍了对DRG神经元亚型的一致识别.
了解DRG神经元异质性对于生理和病理状态,特别是慢性疼痛至关重要.

研究的目的:

整合和综合DRG神经元现有转录组学研究的发现.
批判性地评估不同的scRNA-seq方法及其对DRG神经元分类的影响.
提出对DRG神经元进行分类的标准化框架,并建议未来的研究方向.

主要方法:

对scRNA-seq研究的综合文献综述集中在DRG神经元上.
在不同研究中使用的分类策略的分析和比较.
讨论DRG分析的各种scRNA-seq技术的优点和局限性.

主要成果:

基于转录基因数据识别多样化的DRG神经元群.
突出了当前DRG神经元分类方案中的不一致性.
总结DRG神经元在生理和慢性疼痛条件下的分子和细胞特征.

结论:

需要对DRG神经元分类采取统一的方法,以推进体感官系统研究.
未来的研究应该集中在分子,细胞和神经网络层面,以获得全面的理解.
标准化scRNA-seq分析将改善对疼痛机制和治疗点的研究.

关键词:

背部根部的角质组织.神经损伤的神经损伤疼痛疼痛疼痛疼痛感官处理感官处理一个单细胞RNA-seqq.翻译学翻译学翻译学翻译学

更多相关视频

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

相关概念视频

Somatosensation

Somatosensation

The somatosensory system relays sensory information from the skin, mucous membranes, limbs, and joints. Somatosensation is more familiarly known as the sense of touch. A typical somatosensory pathway includes three types of long neurons: primary, secondary, and tertiary. Primary neurons have cell bodies located near the spinal cord in groups of neurons called dorsal root ganglia. The sensory neurons of ganglia innervate designated areas of skin called dermatomes.

Nervous Tissue: Neuron Types

Nervous Tissue: Neuron Types

Neurons, the fundamental units of the nervous system, can be classified based on both their structural and functional characteristics.
Structurally, neurons are categorized into three main types: multipolar, bipolar, and unipolar (or pseudounipolar). Multipolar neurons, which are the most common type in the brain and spinal cord, as well as all motor neurons, possess multiple dendrites and a single axon.
Bipolar neurons, on the other hand, have one primary dendrite and one axon. They are...

Overview of Somatic Sensory Pathways

Overview of Somatic Sensory Pathways

Somatic sensory or somatosensory pathways refer to the neural pathways that carry information related to touch, pressure, pain, temperature, and proprioception from the skin, muscles, tendons, and joints to the brain. These pathways involve several stages of processing and integration of sensory information.
The somatosensory system is divided into three main pathways: the dorsal (or posterior) column-medial lemniscus, spinothalamic (or anterolateral), and spinocerebellar pathways.
The dorsal...

Sensory Perception: Organization of the Somatosensory System

Sensory Perception: Organization of the Somatosensory System

The somatosensory system is the central and peripheral nervous system component that senses and processes touch, pressure, pain, temperature, and body position or proprioception. The process of sensation takes place at three levels:
The receptor level:
The receptor level is the first stage of sensation. It involves the detection of a stimulus by specialized sensory receptors. The stimulus must arrive within the receptor's receptive field. Next, the receptor converts the energy of the...

Somatosensory, Motor, and Association Cortex

Somatosensory, Motor, and Association Cortex

The somatosensory cortex in the parietal lobes is crucial for interpreting sensory data such as touch, temperature, and proprioception. The somatosensory cortex, situated in the parietal lobes, plays a vital role in interpreting sensory information like touch, temperature, and proprioception—awareness of body position. This specialized brain region features an organized structure wherein neurons at the top primarily process sensations originating from the lower body. In contrast, those at...

Major Somatic Sensory Pathways

Major Somatic Sensory Pathways

Sensory impulses related to touch, pressure, vibration, and proprioception from various body parts, such as the limbs, trunk, neck, and posterior head, travel to the cerebral cortex through the posterior column-medial lemniscus pathway. The pathway’s name derives from the two white-matter tracts that convey the impulses: the spinal cord's posterior column and the brainstem's medial lemniscus. First-order sensory neurons extend their axons into the spinal cord, forming the...