在牛奶加工过程中甲素D的行为及其对新鲜奶酪模型中的苦味的贡献
Gudrun Horstmann1, Johannes Schäfer2, Melanie Rosenberger2
1Department of Biotechnology and Enzyme Science, University of Hohenheim, Institute of Food Science and Biotechnology, Garbenstrasse 25, 70599 Stuttgart, Germany.
Journal of dairy science
|June 2, 2023
概括
牛甲素D对热很敏感,高温会使其失活. 在奶酪中的残留活性没有引起苦味,表明它.
科学领域:
- 食品科学 食品科学 食品科学
- 酶学 是一种酶学.
- 乳制品化学 乳制品化学
背景情况:
- 牛甲素D是一种牛奶内酶.
- 它在乳制品中的苦形成中的作用尚不清楚.
- 在乳制品加工过程中,热不活化至关重要.
研究的目的:
- 为了研究牛甲素D的热无活化.
- 为了评估残留cathepsin D活性对模型新鲜奶酪的味道的影响.
- 为了确定cathepsin D是否有助于苦形成.
主要方法:
- 脱脂牛奶中甲素D的热失活动力学.
- 感官评价 (三角测试) 的模型新鲜奶酪与cathepsinD.
- 对苦的HPLC-双重质谱 (MS) 分析.
主要成果:
- 卡塞普辛D显示对热的敏感性,在90°C以上完全无活化.
- 消毒 (72°C20秒) 留下了残留活性 (~20%).
- 没有显著的味道差异或可检测的苦在奶酪加素D.
结论:
- 在高温处理过程中,甲素D被有效地非活性化.
- 在模型新鲜奶酪中的残留甲素D活性不会导致苦味.
- 单独的cathepsin D不太可能导致牛奶蛋白中的苦形成.
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