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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
Patch Clamp01:18

Patch Clamp

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
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Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
758
Underflow Gates01:30

Underflow Gates

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Underflow gates are vital for controlling water flow in irrigation canals. The three main types of underflow gates — vertical, radial, and drum gates — serve different purposes while ensuring effective flow management. Vertical gates move up and down, generating a free-flowing water jet; radial gates pivot to regulate the flow; and drum gates rotate for precise adjustments. The flow through these gates is influenced by downstream conditions, resulting in free or drowned outflow.Free and...
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相关实验视频

Updated: Jul 28, 2025

In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

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在NOTCH4中,

Bin Zhang1, Shaowei Dong2, Jian Wang1

  • 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong, PR China.

Nature communications
|June 2, 2023
PubMed
概括
此摘要是机器生成的。

NOTCH4ΔL12_16突变通过降低HES1表达,使肺腺癌患者对EGFR-TKI治疗敏感. 针对NOTCH4-HES1通路可能会克服LUAD中的EGFR-TKI抵抗.

更多相关视频

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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相关实验视频

Last Updated: Jul 28, 2025

In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

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4.2K
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 皮肤上生长因子氨酸激酶抑制剂 (EGFR-TKI) 耐药性是肺腺癌 (LUAD) 治疗中的一个重要障碍.
  • 了解抵抗机制对于开发有效的治疗策略至关重要.

研究的目的:

  • 研究NOTCH4突变在LUAD中的EGFR-TKI耐药性的作用.
  • 确定克服EGFR-TKI耐药性的潜在治疗点.

主要方法:

  • 在EGFR-TKI敏感和耐药的LUAD患者中分析NOTCH4突变.
  • 功能性研究涉及 LUAD 细胞中 NOTCH4 突变的外源诱导.
  • 研究NOTCH4-HES1信号通路及其与p-STAT3.3的相互作用.
  • 使用药理抑制剂和小干扰RNA (siRNAs) 抑制NOTCH4-HES1通路.

主要成果:

  • 在EGFR-TKI敏感的LUAD患者中观察到NOTCH4 L12_16删除突变 (NOTCH4ΔL12_16) 的频率增加.
  • NOTCH4ΔL12_16对EGFR-TKI抗性LUAD细胞敏感的EGFR-TKI细胞的外部表达.
  • NOTCH4ΔL12_16突变降低了细胞内NOTCH4 (NICD4),导致通过改变的促进体与p-STAT3.3结合来减少HES1的表达.
  • 抑制NOTCH4-HES1通路逆转了LUAD细胞中的EGFR-TKI耐药性.

结论:

  • NOTCH4ΔL12_16突变通过下调HES1表达,使LUAD对EGFR-TKI敏感.
  • 向NOTCH4-HES1通路是克服LUADEGFR-TKI耐药性的潜在策略.
  • 这一发现为管理抗EGFR-TKI治疗的LUAD患者提供了一种新的治疗方法.