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从活跃和不活跃的怀孕个体在人类胎盘中对霍夫尔细胞极化和VEGF局部化的表征

Alexandra D Goudreau¹, Catherine Everest¹, Layli Tanara²

¹Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada.

Physiological reports

|June 3, 2023

概括

怀孕期间的体力活动会影响霍夫尔细胞 (HBC) 两极分化,增加CD206+细胞的比例. 这表明与运动相关的妊娠健康益处的潜在机制.

科学领域:

生殖生物学和免疫学.
怀孕和母亲和胎儿的健康
身体活动的细胞机制

背景情况:

身体活动 (PA) 对母亲和胎儿的健康有好处,但潜在的机制尚不清楚.
在健康妊娠中,霍夫尔细胞 (HBC) 存在于CD206+和CD206-表型中,CD206+细胞占主导地位.
血清细胞参与血管生成,PA影响巨细胞的两极分化.

研究的目的:

为了研究身体活动水平和怀孕期间霍夫尔细胞极化之间的关系.
确定哪些霍夫尔细胞表现型表达血管内皮生长因子 (VEGF).

主要方法:

根据PA水平将参与者分为活跃和不活跃的组.
免疫光细胞标记以量化HBCs,CD206+ HBCs和CD206表达.
免疫光同位定位用于识别HBC表型中的VEGF表达.
西部斑点和RT-qPCR测量CD68和CD206蛋白质和mRNA的表达.

主要成果:

两个CD206+和CD206-HBC表达VEGF. 这两个CD206+和CD206-HBC表达VEGF.
在活跃参与者中,CD206+ HBC 的比例更高.
活跃参与者显示CD206蛋白质表达较低,尽管CD206mRNA水平相似.

结论:

身体活动可以调节怀孕期间的霍夫尔细胞两极化.
这些发现表明,在HBC中,可能会有PA介导的CD206的翻译调节.
这项研究提供了有关PA与改善妊娠结果的细胞机制的见解.

关键词:

霍夫巴乌尔细胞是什么?血管新生是因为血管新生.巨细胞的两极分化身体活动身体活动胎盘胎盘胎盘胎盘怀孕怀孕怀孕怀孕怀孕

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