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在膀癌中,CirccHIPK3通过阻断VCP与Beclin 1复合体的结合来负面调节自

Chong Wang^1,2,3, Tiantian Liu^1,2, Jiawei Wang²

¹Department of Urology, The Fifth Affiliated Hospital Sun Yat-Sen University, Zhuhai, 519000, Guangdong, People's Republic of China.

Discover oncology

|June 3, 2023

概括

循环RNA HIPK3 (circHIPK3) 在膀癌中被下调,促进细胞增殖和自. 它抑制了VCP介导的自,这表明它在膀癌的进展中发挥了作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

循环RNA HIPK3 (circHIPK3) 与各种癌症有关.
circHIPK3在调节膀癌中自的特定作用仍然在很大程度上是未知的.
自是一种关键的细胞过程,涉及到细胞生存和死亡.

研究的目的:

阐明 circHIPK3 调节膀癌细胞自的机制.
研究circHIPK3与参与自的结合蛋白的相互作用.
在膀癌中确定circHIPK3的潜在调节途径.

主要方法:

在膀癌组织和细胞中对circHIPK3和与自相关的蛋白质进行定量分析.
在不同的circHIPK3表达水平下评估膀癌细胞的增殖.
调查circHIPK3对VCP/Beclin 1相互作用和自的影响.
分析VCP在稳定Beclin 1和通过ATAXIN-3调节自的作用.

主要成果:

与正常组织相比,膀癌中CirccHIPK3的水平显着较低,随着对应的自蛋白的上调.
循环HIPK3的下调促进了膀癌细胞的增殖,而其过度表达抑制了增殖.
过度表达circHIPK3抑制了自,并破坏了VCP/Beclin 1的相互作用.
发现VCP通过降低ATAXIN-3的调节来稳定Beclin 1并促进自.

结论:

CircHIPK3在膀癌的进展中起着重要作用.
在膀癌细胞中,CirccHIPK3抑制了VCP介导的自.
circHIPK3可能代表膀癌治疗的潜在治疗标.

关键词:

自自是一种自的过程.膀癌是一种癌症.这是循环RNARNA.它们是RNA结合蛋白 (RNA-binding proteins,简称RBPs).这是一个VCPVCP.

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