用肝脏ECM蛋白来功能化的携带氧的微粒的制造,以改善表型的体外三维肝脏组合,功能和反应
Mona Mansouri1, William D Imes2, Owen S Roberts3
1Department of Chemical, Biomolecular, and Corrosion Engineering, The University of Akron, Akron, Ohio, USA.
Biotechnology and bioengineering
|June 3, 2023
概括
这项研究使用释放氧气的微粒和特定的细胞外基质 (ECM) 蛋白来设计3D肝脏球体. 在这些先进的器官模型中,拉米宁-511和拉米宁-521显著改善了肝细胞的功能和组织.
科学领域:
- 生物材料工程 生物材料工程
- 细胞生物学 细胞生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 氧和细胞外基质 (ECM) 生物聚合物对于肝细胞功能至关重要.
- 调整3D肝细胞聚合物的微环境是增强代谢功能的关键.
- 分别使用HepG2和LX-2细胞系作为肝细胞和肝星细胞的模型.
研究的目的:
- 通过增强氧气可用性和ECM配体呈现来协同调整3D肝细胞聚合物的微环境.
- 在工程3D模型中促进人类肝脏的原生代谢功能.
- 研究特定ECM蛋白在调节肝细胞表型中的不同作用.
主要方法:
- 使用微流体制造化 (PFC) 基多微粒 (MPs).
- 使用基于的氧气传感方法评估氧气运输特性.
- 用肝脏ECM蛋白 (纤维素,拉米林-111, -511, -521) 功能化MPs,并用HepG2和LX-2细胞组装复合球体.
主要成果:
- 在培养了功能MP的球体体中观察到增强的肝脏特异性功能和细胞粘附模式.
- 拉米宁-511和拉米宁-521显著改善了肝脏的表型反应,由增加的E-cadherin和文库林表达体现出来.
- 肝细胞和肝星细胞在与胺-511和-521修饰的MPs共同培养时表现出更明显的表型安排.
结论:
- 特定的ECM蛋白质,特别是-511和-521,在工程3D球体内肝细胞的表型调节中发挥着独特的作用.
- 这种方法有助于创建具有明确条件的生理相关器官模型.
- 改善的表型细胞信号增强了3D球形和有机形模型对肝脏研究的相关性.
相关概念视频
01:42The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:26Extracellular Matrix
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
01:30Liver Physiology
The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
Metabolic Regulation:
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Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can also...