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来自Trichoderma virensens的FAD依赖型葡萄糖脱酶的电化学和生物感知特性

Sudarma Dita Wijayanti¹, Franziska Schachinger², Roland Ludwig²

¹Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Wien, Austria; Department of Food Science and Biotechnology, Brawijaya University, Veteran, 65145 Malang, East Java, Indonesia.

Bioelectrochemistry (Amsterdam, Netherlands)

|June 3, 2023

概括

这项研究详细介绍了一种使用Trichoderma virens葡萄糖脱酶 (TvGDH) 来检测马尔的新生物传感器. 工程生物传感器具有高灵敏度和低检测极限的麦芽糖,使其成为传感器应用的前景.

科学领域:

生物化学生物化学
电化学电化学电化学
生物传感器技术技术

背景情况:

来自Trichoderma virens (TvGDH) 的FAD依赖型葡萄糖脱酶 (GDH) 显示出一种不寻常的基质偏好麦芽糖而不是葡萄糖.
TvGDH的电化学特性和作为生物传感器识别元件的潜力具有重大意义.

研究的目的:

为了研究TvGDH的生物电化学特性.
开发和描述一种生物传感器,用于使用固定TvGDH检测马尔.

主要方法:

确定了TvGDH的氧化还原潜力.
使用氧化还原聚合物和交联剂将TvGDH固定在石墨电极上.
测试了生物传感器的性能,使用麦芽糖和其他糖类.

主要成果:

确定TvGDH的氧化还原潜力为-0.268 ± 0.007V与SHE相比.
基于TvGDH的生物传感器的灵敏度为1.7 μA mM-1cm-2,线性范围为0.5-15 mM,对马尔托的检测极限为0.45 mM.
生物传感器显示,与其他糖相比,麦芽糖的明显迈凯利斯-门常数 (KM app) 是最低的 (19.2 ± 1.5 mM),表明特异性高.

结论:

在生物传感器应用中,TvGDH具有有利的氧化还原潜力.
开发的TvGDH生物传感器在检测马尔时具有良好的灵敏度和特异性.
生物传感器检测其他糖类的能力表明干扰的可能性,这需要在实际应用中考虑.

关键词:

电压计是指对电压的测量.这是一种FAD-依赖的葡萄糖脱酶.转基因的氧化还原酶 (GMC oxidoreductases) 是一种马尔托斯生物传感器是一种生物传感器.聚合物的聚合物.三真皮病毒 (Trichoderma virens) 是一种真皮病毒.

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