铁素TRXo1通过PYR1氧化还原调节参与ABA感知
Sabrina De Brasi-Velasco1, Antonio Sánchez-Guerrero1, Mari-Cruz Castillo2
1Department of Stress Biology and Plant Pathology, CEBAS-CSIC, E-30100, Murcia, Spain.
Redox biology
|June 3, 2023
概括
铁素o1 (TRXo1) 通过调节PYR1受体来调节酸 (ABA) 信号传输.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酸 (ABA) 对于植物的生长,发育和应激反应至关重要.
- ABA信号传递涉及PYR/PYL/RCAR受体,这些受体抑制PP2C酸酶,从而激活SnRK2激酶.
- 铁素 (TRXs) 是细胞氧化还原平衡的关键调节剂,在各种细胞过程中发挥作用.
研究的目的:
- 为了在核中确定 thioredoxin o1 (TRXo1) 的新点.
- 研究TRXo1在ABA信号元件的氧化还原调节中的作用.
- 阐明TRXo1与PYR1受体相互作用和修改的机制.
主要方法:
- 使用亲和色谱,Dot-blot,共免疫沉和双分子光补充试验来识别PYR1作为TRXo1目标.
- 对重组HisAtPYR1 (野生类型和突变) 的研究研究了氧化还原调节和寡合体状态的变化.
- 在体内实验中评估了在ABA治疗下野生类型和Attrxo1突变植物中的PYR1寡合化.
主要成果:
- 确定PYR1是TRXo1.1的一个新核目标.
- PYR1的氧还原调节涉及其寡合状态的变化,Cys30和Cys65残留物至关重要.
- TRXo1有效地减少了氧化,不活跃的PYR1,恢复了它抑制HAB1酸酶的能力.
- 活体中PYR1的寡合化是依赖氧化还原的,并且在Attrxo1突变体中显示出差异性模式.
结论:
- TRXo1通过氧化还原修饰直接调节PYR1受体.
- 这种氧化还原调节会影响PYR1与PP2Cs的相互作用,并影响ABA信号传递.
- 这些发现揭示了先前未被描述的ABA信号调节的机制.
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