转基因小鼠缺乏一个或两个胡卜素裂解酶的莱科积累
Madelyn J Bradley1, Molly Black2, Joseph R Arballo1
1Division of Nutritional Sciences, University of Illinois Urbana Champaign, Urbana, IL.
The Journal of nutrition
|June 3, 2023
概括
β-胡卜素氧化酶2 (BCO2) 是小鼠中溶烯分解的主要酶. 百合素积聚在肝脏线粒体中,并影响维生素A信号通路.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- β-胡卜素氧化酶1 (BCO1) 和β-胡卜素氧化酶2 (BCO2) 是负责哺乳动物中胡卜素代谢的关键酶.
- 了解它们的特定作用对于阐明营养吸收和利用途径至关重要.
研究的目的:
- 为了确定BCO1和BCO2在小鼠中烯代谢中的相对贡献.
- 为了研究烯对野生型 (WT) 鼠的肠道内基因表达的影响.
主要方法:
- 使用了WT,Bco1-/-,Bco2-/-,和双淘汰赛 (DKO) 的小鼠,每天给他们注射二烯或两周的载体控制.
- 使用高性能液态染色学评估了烯度和同位素分布.
- 通过RT-PCR评估了食维生素A对柳科吸收和肠道基因表达的影响.
主要成果:
- 肝脏在所有基因型中积累了94-98%的烯,Bco1-/-小鼠与其他组相比显示了大约一半的肝脏烯水平.
- 线粒体中毒素含量在肝细胞中显著增加 (3至5倍),不论基因型或性别.
- 缺乏维生素A的饮食中的小鼠积累了更多的肝胆,并伴随着肠特异性家庭盒 (ISX) 转录因子的上调.
结论:
- 在小鼠中,BCO2似乎是主要的酶,负责烯裂变.
- 利科在肝脏线粒体中被优先丰富,独立于特定的BCO1/BCO2基因型.
- 饮食中使用的烯对小鼠肠道中的维生素A信号通路产生积极影响.
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