来自Chlamydomonas reinhardtii的三种PSI-LHCI结构表明,静止状态被铁素重新激活
Christoph Gerle1, Yuko Misumi2, Akihiro Kawamoto2
1Life Science Research Infrastructure Group, RIKEN SPring-8 Center, Kouto, Hyogo, Japan; Laboratory for Protein Crystallography, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
Biochimica et biophysica acta. Bioenergetics
|June 3, 2023
概括
在Chlamydomonas reinhardtii中,光系统I (PSI) 超级复合体表现出一种静止状态,其中叶绿素减少,电子捐赠者停靠. 氧化铁素结合激活了这种PSI-LHCI复合体.
科学领域:
- 光合作用研究研究光合作用.
- 结构生物学是结构生物学.
- 藻类生物化学 藻类生物化学
背景情况:
- 在结构上,光系统I (PSI) 和其光收获复合体 (LHCI) 在Chlamydomonas reinhardtii中得到了很好的研究.
- 然而,可溶性结合伙伴的结构性表征仍然不那么先进.
研究的目的:
- 为了研究来自Chlamydomonas reinhardtii的光系统I-LHCI超级复合物的结构基础.
- 描述可溶性绑定伙伴及其在规范PSI活动中的作用.
主要方法:
- 在X射线晶体学.
- 单粒子冷电子显微镜 (冷电子显微镜)
主要成果:
- X射线结构显示,LHCI带的光侧缺少六种叶绿素,这可能会影响激发转移.
- 低温电磁探测器在电子转移点附近发现了额外的密度,这些密度在氧化铁素结合后消失了.
- 这些发现表明,PSI-LHCI静止状态具有减少的叶绿素和对接的电子捐赠者/监管伙伴.
结论:
- 建议对PSI-LHCI超级综合体进行一种新的静止状态.
- 这种静止状态的特点是减少叶绿素含量和预定位置的电子转移元件.
- 建议氧化铁素的可用性可以触发向活性PSI-LHCI形式的过渡.
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