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在生物膜分散和拆卸后,细菌群体的独特动态和疾病传播
Yeping Ma1, Yanlin Deng2, Haojun Hua2
1Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.
The ISME journal
|June 3, 2023
概括
生物膜分散是复杂的:化学分散释放单个不能殖民的细胞,而酶分解释放聚合物,有效地感染宿主,影响疾病传播.
科学领域:
- 微生物学 微生物学
- 病变的发生和发病.
- 生物膜的动力学
背景情况:
- 生物膜的分散对于微生物的生存,传播和感染传播至关重要.
- 了解生物膜分散的机制和后果是有限的.
- 从生物膜释放后的细菌异质性使研究复杂化.
研究的目的:
- 研究生物膜分散的独特时空动态及其对重新殖民和疾病的影响.
- 为了比较Pseudomonas aeruginosa生物膜的化学诱导分散 (CID) 和酶分解 (EDA).
- 分析细菌在殖民和宿主感染中传播后行为的作用.
主要方法:
- 开发一种新的3D细菌生物膜分散再重新定居 (BDR) 微流体模型.
- 在CID和EDA期间观察Pseudomonas aeruginosa生物膜动态.
- 评价细菌的重新定居能力和感染潜力在肺球体和Caenorhabditis elegans模型中.
主要成果:
- CID导致单细胞释放,需要bdlA基因和鞭毛细胞,但这些细胞无法殖民新表面或感染宿主.
- 涉及外聚糖 (Psl) 降解的EDA释放不动聚合物,有效地重新定居表面并引起感染.
- 分散后细菌的独特行为导致殖民和疾病传播的结果相反.
结论:
- 生物膜分散机制显著影响细菌的生存和病原性.
- 化学诱导的分散和酶分解对重新殖民和感染有不同的后果.
- 细菌群体的异质性和分散后的行为是微生物物种生存和疾病传播的关键因素.
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