在垂体腺瘤中PVT1和NEAT1 lncRNAs的失调
Soudeh Ghafouri-Fard1, Farzaneh Abbasi2, Amir Nicknam3
1Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pathology, research and practice
|June 4, 2023
概括
这项研究发现,NEAT1和PVT1长非编码RNAs (lncRNAs) 在非功能性垂体腺瘤 (NFPAs) 中失调. 这些lncRNAs可能在NFPAs的发展中发挥作用,表明潜在的诊断相关性.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 垂体腺瘤是缓慢生长的瘤,起源于前垂体.
- 长非编码RNAs (lncRNAs) 参与调节与瘤发展相关的细胞过程.
- 已知PVT1,TUG1,MALAT1,NEAT1和GAS5等特定的lncRNAs可以影响细胞增殖,细胞亡,分化和细胞周期.
研究的目的:
- 为了研究PVT1,TUG1,MALAT1,NEAT1和GAS5在垂体腺瘤组织中的表达水平,与相邻的非癌细胞组织相比.
- 确定这些lncRNAs在垂体瘤中的相关性及其作为诊断标记物的潜力.
- 探索NEAT1和PVT1在非功能性垂体腺瘤 (NFPAs) 病变发生中的特定作用.
主要方法:
- 在垂体腺瘤和相邻的非癌性组织样本中对lncRNA表达水平的定量评估.
- 统计分析以比较瘤和对照组之间的表达比率和P值.
- 对PVT1和NEAT1的灵敏度和曲线下的面积 (AUC) 值的评估,以评估它们对NFPAs的诊断潜力.
主要成果:
- 与对照组相比,NEAT1的表达在整个垂体腺瘤组织 (表达比率=7.06,P=0.02) 和特别是在NFPA样本 (表达比率=8.5,P=0.04) 中显著升高.
- 无论是PVT1还是NEAT1,都显示出高灵敏度值 (分别为0.84和0.90) 来区分NFPA与非癌性组织.
- 然而,PVT1 (0.63 ± 0.04) 和NEAT1 (0.58 ± 0.04) 的AUC值不被认为足以用于最终诊断.
结论:
- 在非功能性垂体腺瘤 (NFPA) 中,NEAT1和PVT1 lncRNAs显著失调.
- 这些发现表明NEAT1和PVT1在NFPAs病变发生过程中的潜在作用.
- 虽然显示出诊断潜力,但需要进一步的研究来验证NEAT1和PVT1作为NFPA的可靠诊断标记.
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