宿主-微生物相互作用和病原体排斥由易聚合的表面层蛋白质Lactobacillus helveticus中介
Ritu Choudhary1, Kumar Siddharth Singh1, Sonu Bisht1
1Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, (India).
International journal of biological macromolecules
|June 4, 2023
概括
再组合的Lactobacillus helveticus表面层蛋白 (SlpH) 在高产量中产生. SlpH与肠道组织结合并抑制病原体的粘附,显示出作为治疗剂的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 益生菌 益生菌 益生菌
背景情况:
- 益生菌表面层蛋白 (Slps) 对于细菌粘附于宿主细胞至关重要.
- 了解Slp函数受到低本地产量和自我聚合的限制.
- 乳杆菌 helveticus NCDC 288 Slp (SlpH) 是一个功能研究的目标.
研究的目的:
- 为了实现生物活性SlpH.的高产重组表达和净化.
- 描述SlpH.的生物物理特性和宿主结合能力.
- 评估SlpH作为对肠道病原体的预防或治疗剂的潜力.
主要方法:
- 来自Lactobacillus helveticus的SlpH的重组表达和净化 NCDC 288.
- 循环二极化谱法用于确定蛋白质结构和稳定性.
- 在人体肠道组织,Caco-2细胞和粘液的体外结合试验.
- 排斥和竞争测试以评估抑制肠毒性大肠杆菌和沙门氏菌的粘附. Typhimurium.
主要成果:
- 实现了生物活性SlpH (45 kDa,pI 9.4) 的高产净化.
- SlpH具有富含β链的结构,低pH耐受性,并与肠道组织,Caco-2细胞和粘真菌结合.
- SlpH显著抑制了大肠杆菌和沙门氏菌Typhimurium粘附于Caco-2细胞 (70-76%的抑制).
结论:
- 再组合的SlpH生产克服了本地产量限制.
- 由于其结合性和抑制性质,SlpH显示出作为预防性或治疗性抗肠道病原体的显著潜力.
- 在胃肠道条件下SlpH的稳定性进一步支持其治疗潜力.
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