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高价值酸生物合成使用模块化设计和在工程Escherichia coli底盘中磨碎的咖啡

Sefli Sri Wahyu Effendi¹, I-Son Ng¹

¹Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.

Bioresource technology

|June 4, 2023

概括

工程化大肠杆菌有效地从廉价的前体中产生酸 (FA),实现高产量和转化率. 这种绿色生物合成方法为生产这种有价值的芳香化合物提供了具有成本效益和节省时间的替代方案.

科学领域:

合成生物学合成生物学
代谢工程是代谢工程.
绿色化学是一种绿色化学.

背景情况:

酸 (FA) 是一种有价值的芳香化合物,具有显著的药理动力学特性.
目前的FA生物合成方法往往耗时且昂贵.
微生物宿主为可持续生产高价值化学品提供了一个有希望的平台.

研究的目的:

开发一种时间和成本效益高的酸 (FA) 生物合成方法.
为了设计大肠杆菌以有效地生产FA.
为了利用廉价的前体用于FA合成.

主要方法:

使用模块化设计,人工重建大肠杆菌中FA生物合成途径.
对大肠杆菌菌株进行有效的p-coumaric acid (pCA) 和FA合成的查.
通过结合氨酸载体,异质子适应,pCA同载体利用和通过体内再生丰富FADH2辅因子池来增强模块化设计.
优化培养条件. 培养条件的优化.

主要成果:

在48小时内实现了972.6mg/L的非凡的FA产量,转化率为89.4%.
成功地证明了使用来自消耗咖啡粉的廉价前体用于FA生物合成.
克服了以前的FA生产方法的耗时限制.

结论:

工程化大肠杆菌菌株为ferulic acid (FA) 生产提供了一个高效和经济的平台.
这项研究建立了一种新的,可持续的FA生物合成方法,利用废物衍生的前体.
开发的方法大大减少了生产时间和成本,为工业应用铺平了道路.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.FADH(2) 的再生过程.费鲁酸是什么费鲁酸是什么费鲁酸是什么模块化设计的模块化设计消耗的咖啡粉是花费的运输基因是运输基因的组成部分.

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