代谢工程的代谢工程
Cui Zhao1,2,3, Xi-Hui Wang1,2,3, Xin-Yao Lu1,2,3
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
ACS synthetic biology
|June 5, 2023
概括
这项研究对Candida glycerinogenes进行了基拉尼生产的工程设计,从基纤维素生物质中获得高位数. 开发的系统将碳流转向,使这种有价值的芳香化合物的可持续合成成为可能.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 工业生物技术 工业生物技术
背景情况:
- 格拉尼奥尔是一种有价值的天然产品,广泛用于芳香行业.
- 现有的生产方法面临来自内源代谢途径的竞争,例如酵母菌的ergosterol途径.
- 从可再生资源中可持续生产日醇是非常可取的.
研究的目的:
- 开发一种转录因子介导的系统,用于自主调节厄戈斯特醇代谢.
- 为了将碳流转向Candida glycerinogenes中的geraniol合成.
- 为了实现从基纤维素生物质中可持续生产日醇.
主要方法:
- 设计了一种转录因子介导的ergosterol反系统,以调节ergosterol代谢.
- 修改了对厄戈斯特醇反应的促进剂和优化的转录因子表达.
- 在Candida glycerinogenes中构建和优化了一条氧化糖同化通路,激活酸通路.
主要成果:
- 通过代谢工程和促进物优化,通过代谢工程和促进物优化实现了531.7毫克L-1的基拉尼标位.
- 成功地从林氏纤维素生物质 (模拟的小麦和甘包水解剂) 制造出基拉尼奥尔.
- 在5L生物反应器中获得高基拉尼标位 (高达1091.6毫克L-1在葡萄糖上) 和产量 (高达59.1毫克g-1DCW).
结论:
- 坎迪达糖原体可以被设计为高效的日醇生产.
- 开发的转录因子介导系统有效地重定向代谢流量,以增强基拉尼奥尔合成.
- 这项研究表明,从纤维素生物质中生产日拉尼和其他单烯的潜力,提供了一个可持续的生物生产平台.
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