布林西多福维尔是一种强大的复制抑制剂,可以对抗非洲猪瘟病毒

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Shibang Guo^1,2, Yibo Zhang^1,2, Zhankui Liu^1,2

¹National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.

Emerging microbes & infections

|June 5, 2023

概括

布林西多福维尔在对抗非洲猪瘟病毒 (ASFV) 感染方面表现有前途. 这种抗病毒剂显著减少了ASFV的复制,并保护猪免受致命的结果,为疾病控制提供了潜在的新策略.

科学领域:

兽医病毒学兽医病毒学
传染性疾病传染性疾病
药物发现药物发现药物发现

背景情况:

非洲猪瘟病毒 (ASFV) 对全球猪业构成重大威胁,目前没有安全有效的疫苗或治疗方法.
由于缺乏有效的对策,迫切需要开发针对ASFV的新型抗病毒策略.

研究的目的:

使用激酶抑制剂库识别安全有效的抗病毒化合物,对抗非洲猪瘟病毒 (ASFV).
评估已识别的化合物在体外和体内用于预防和控制ASFV感染的疗效.

主要方法:

使用感染ASFV的初级猪膜巨细胞对酶抑制剂库的查.
在体外测试以确定候选化合物的抑制度 (IC50) 和细胞毒性 (CC50).
在体内猪挑战实验中评估保护效果,病毒载量减少和本病理变化.

主要成果:

六种化合物抑制ASFV感染超过90%,其中布林西多福维尔显示最佳疗效 (IC50 = 2.76nM,CC50 = 58μM).
布林西多福维尔以剂量依赖的方式降低了ASFV复制,病毒标位和结构蛋白表达,干扰了入侵后的阶段.
在体内,布林西多福维尔保护猪免受ASFV致命性,减少病毒载量和流失,并减轻相关组织损伤.

结论:

布林西多福维尔显示出作为预防和控制非洲猪瘟病毒 (ASFV) 感染的有效药物的巨大潜力.
进一步的研究是有必要的,以充分探索布林西多福维尔的治疗应用在ASFV.

关键词:

非洲猪瘟病毒病毒中国巴马小猪的小猪抗病毒活动的抗病毒活性.这是一个brincidoofovir.激酶抑制剂图书馆激酶抑制剂图书馆

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