水素调节细胞通过真空形成的圆形化,在内皮细胞转化为造血细胞的过渡期间
Yuki Sato1, Mugiho Shigematsu1, Maria Shibata-Kanno1
1Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-0054, Japan.
概括
水素1 (AQP1) 在内皮细胞转化为血液细胞的过程中驱动血造干细胞 (HSC) 的产生. 水流入真空孔会导致细胞圆和脱落,这对于高血压细胞的形成至关重要.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 血液形成 血液形成 血液形成
背景情况:
- 内皮转化为造血细胞的转变 (EHT) 对于产生造血细胞干细胞 (HSC) 至关重要.
- 在EHT过程中,血源性内皮细胞 (HECs) 经历了形态变化,圆和脱离背部大动脉.
- 在HEC中驱动这种非典型细胞圆化的精确机制在很大程度上是未知的.
研究的目的:
- 阐明EHT期间形态变化的基础分子机制.
- 为了研究水素素在细胞形状调节中的作用,在HSC生成过程中.
主要方法:
- 在鸟类HEC中观察过渡性真空球形成和水素1 (AQP1) 局部化.
- 在非HEC中过度表达AQP1,以评估其对细胞形态和脱落的影响.
- 通过CRISPR/Cas9基因编辑,研究HEC中AQP的功能.
主要成果:
- 在EHT期间,在鸟类HEC中形成过渡性,大的真空孔,AQP1局部化到真空孔和血膜.
- 在非HEC中,AQP1过度表达诱导了真空孔扩张,细胞圆和脱落,模仿了EHT.
- 通过CRISPR/Cas9破坏AQP功能,导致HECs的形态EHT受损.
结论:
- 在EHT期间,血液细胞与内皮细胞的形态分离由水流入真空管调节,由AQP1.1调节.
- 这项研究揭示了一种由适应水的细胞反应驱动的细胞形态发生的新机制.
- 这些发现提供了关于细胞和组织形态发生的更广泛原则的见解.
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