循环PLXNB2通过miR-654-3p/p调节急性髓性白血病的进展
Jiao Wang1, Cuiping Wu2, Weilun Zhou1
1Department of Hematology, The First Affiliated Hospital of Hainan Medical University, Haikou, People's Republic of China.
Hematology (Amsterdam, Netherlands)
|June 5, 2023
概括
循环RNA素B2 (circPLXNB2) 在急性髓性白血病 (AML) 中高度表达,并促进癌症的进展. 针对circPLXNB2/microRNA-654-3p/cyclin D1轴可能为AML提供一种新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 急性髓性白血病 (AML) 是一种流行的血液性恶性瘤.
- 循环RNAs (circRNAs) 是癌症生物学中基因表达的新兴调节者.
- 确定AML的新诊断和治疗点至关重要.
研究的目的:
- 为了研究circRNA Plexin B2 (circPLXNB2) 在AML中的作用.
- 阐明circPLXNB2在AML进展中的作用背后的分子机制.
- 评估circPLXNB2作为AML的潜在治疗点.
主要方法:
- 定量实时PCR (qRT-PCR) 用于测量circPLXNB2,microRNA-654-3p (miR-654-3p) 和cyclin D1 (CCND1) 的表达水平.
- 功能性检测包括细胞增殖 (CCK-8,EDU),细胞亡和细胞循环分析.
- 双露西法酶记者测定证实circPLXNB2,miR-654-3p和CCND1.1之间的相互作用.
主要成果:
- 在AML患者和细胞中,circPLXNB2显著上调,并且表现出比线性PLXNB2更高的稳定性.
- 敲除circPLXNB2抑制了AML细胞增殖,诱导了细胞亡和改变了细胞周期分布.
- circPLXNB2 作为 miR-654-3p 的分子海绵,反过来针对 CCND1,从而调节 AML 的进展.
结论:
- circPLXNB2在通过circPLXNB2/miR-654-3p/CCND1通路调节AML瘤进展方面发挥着至关重要的作用.
- circPLXNB2代表了急性髓性白血病治疗的有前途的新型治疗标.
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