共同文化的共同文化
Benedict Ryan Lukito1, Nurhidayah Basri1, Aaron Thong1
1Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Level 6, Nanos, Singapore 138669, Republic of Singapore.
Journal of agricultural and food chemistry
|June 5, 2023
概括
与Meyerozyma guilliermondii共同培养Kluyveromyces marxianus,并使用现场产品回收 (ISPR) 显著提高了2-乙醇 (2-PE) 产量. 这种方法提高了选择性和体积生产率,同时减少了副产品的形成.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 2-乙醇 (2-PE) 是一种有价值的芳香化学物质,由Kluyveromyces marxianus生产.
- 提高2-PE生物生产的选择性和生产率仍然是一个挑战,通常是由副产品形成的复杂化,如乙烯酸 (PEA).
- 在现场产品回收 (ISPR) 技术,如使用吸收性聚合物,可以帮助生物生产,但可能会影响产品选择性.
研究的目的:
- 研究K. marxianus与另一种酵母菌种共同培养的有效性,并与ISPR一起用于增强2-PE生物生产.
- 评估共同培养和ISPR对2-PE和副产品PEA之间的选择性的影响.
- 为了评估与ISPR纯培养发酵相比,对体积生产率的影响.
主要方法:
- K. marxianus (MUCL 53775) 的发酵,直接加入吸收聚合物Hytrel3548,用于ISPR.
- 在类似的ISPR条件下,K. marxianus (MUCL 53775) 与Meyerozyma guilliermondii (MUCL 28072) 的共同培养发酵.
- 对2-PE和PEA积累,选择性和体积生产力的分析.
主要成果:
- 使用ISPR对K. marxianus的纯培养发酵有利于PEA的积累,导致2-PE选择性低 (13%).
- 在ISPR条件下,K. marxianus与M. guilliermondii的共同培养显著限制了PEA的产生,将2-PE的选择性提高到90%.
- 与纯种植ISPR方法相比,共同培养方法提高了85%的体积生产率.
结论:
- 在ISPR的双相发酵系统中与M. guilliermondii共同培养K. marxianus有效调节生物生产,提高2-PE的选择性和生产力.
- 微生物共同培养和ISPR之间的相互作用对于控制2-PE和PEA形成之间的平衡至关重要.
- 这种协同方法显示出其他高价值分子的生物生产的希望,使用不同的菌株组合和ISPR技术.
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