相关实验视频

Updated: Jul 28, 2025

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Published on: September 5, 2019

由脂肪酸氧化为燃料的氧化酸化使白血病干细胞对寒冷敏感

Emmanuel Griessinger^1,2, Diego Pereira-Martins¹, Marielle Nebout²

¹Department of Experimental Hematology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.

Cancer research

|June 5, 2023

概括

低温可以通过向它们对线粒体氧化酸化 (OxPhos) 的依赖来选择性地杀死急性髓性白血病干细胞 (LSCs). 这种与脂肪酸氧化 (FAO) 相关的寒冷敏感性为AML研究提供了新的治疗脆弱性和潜在的生物标志物.

科学领域:

细胞生物学细胞生物学
癌症新陈代谢癌症新陈代谢
血液学血液学血液学

背景情况:

在急性髓性白血病 (AML) 中,白血病干细胞 (LSC) 通常依赖于线粒体氧化酸化 (OxPhos) 才能生存.
脂肪酸氧化 (FAO) 是一种关键的代谢途径,在某些AML细胞中为OxPhos提供燃料,特别是那些耐化学疗的AML细胞.

研究的目的:

在AML LSC中识别新的治疗漏洞.
为了研究细胞代谢在寒冷敏感性中的作用.
探索寒冷敏感性作为AML的潜在生物标志物.

主要方法:

将AML细胞和正常的造血干细胞暴露在低温 (4°C) 中.
代谢概况 (OxPhos与糖解依赖).
脂质和蛋白质分析以评估膜组成.

主要成果:

低温 (4°C) 选择性地诱导AMLLLSC中的细胞死亡,其中含有活性OxPhos,特别是那些由粮农组织提供燃料的.
依赖糖解的白血病细胞对冷引起的细胞死亡具有抗性.
强迫糖溶性细胞利用OxPhos使它们对寒冷敏感.
OxPhos改变了血的组成,增加了对冷气引起的膜通透性的敏感性.

结论:

在体温下的稳定状态能量代谢决定了AML LSC对寒冷的敏感性.
感冒敏感性是依赖OxPhos的AML LSCs的一个新脆弱性.
寒冷敏感性可能作为AMLLLSCs的生物标志物.
应避免在4°C的AML样本的实验性储存,以防止人工细胞死亡.

更多相关视频

Modeling Chemotherapy Resistant Leukemia In Vitro

Modeling Chemotherapy Resistant Leukemia In Vitro

Published on: February 9, 2016

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

相关概念视频

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...