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在铁获取和Riemerella的病原性中FeoAB的功能性表征 anatipestifer

Mi Huang^1,2,3,4, Mengying Wang^1,2,3,4, Yan Feng^1,2,3,4

¹Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, China.

Microbiology spectrum

|June 5, 2023

概括

Riemerella anatipestifer使用Feo系统吸收铁,对于生长和殖民至关重要. 破坏这个系统会减少铁的进口,影响毒性,增加对氧化应激的抵抗力.

科学领域:

微生物学微生物学
细菌生理学细菌生理学
病变的发生和发病.

背景情况:

瑞美瑞拉 (Riemerella anatipestifer) 需要铁才能生长.
在R. anatipestifer中,铁的吸收机制,特别是Feo系统,尚未完全理解.
铁的获取对于细菌毒性和氧化应激耐药性至关重要.

研究的目的:

为了描述Feo系统在R. anatipestiferATCC 11845.5铁进口中的功能.
研究Feo系统在R. anatipestifer对铁限制和氧化应激反应中的作用.
为了确定Feo系统对R. anatipestifer在体内殖民能力的影响.

主要方法:

在R. anatipestiferATCC 11845.5中的feoA,feoB和feoAB基因的遗传破坏.
在铁有限条件下的增长曲线分析.
对斯特尼格林和过氧化的敏感性评估.
使用毛皮调节器对铁和其他金属对feoA和feoB基因调节的分析.
在小的体内殖民化研究.

主要成果:

由于细胞内铁的减少,Feo系统的破坏 (ΔfeoA,ΔfeoB,ΔfeoAB菌株) 在铁有限的条件下损害了细菌的生长.
这种 ΔfeoAB 菌株对斯特尼格林和过氧化的敏感性降低.
feoA和 feoB的表达是通过Fur进行铁调节的,并由MnCl2,ZnSO4和CuCl2.2诱导.
在子中,ΔfeoAB菌株显著减少了血液,肝脏和大脑的殖民.

结论:

在R. anatipestifer.中,FeoAB系统对于铁铁的获取至关重要.
在R. anatipestifer的毒性和殖民化中,FeoAB起着至关重要的作用.
Feo系统可能参与感知和响应,和铜应力,表明金属运输通路之间的交叉交谈.

关键词:

在FeoAA.在 FeoBB 里面.里梅瑞拉是一种比较有害的虫害.铁铁运输铁铁运输铁铁运输

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