Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Anoxygenic Phototrophic Bacteria01:28

Anoxygenic Phototrophic Bacteria

63
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
63
Microbial Nutrition01:28

Microbial Nutrition

117
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
117
Bacterial Phylum Bacteroidota01:26

Bacterial Phylum Bacteroidota

50
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
50
Microbial Fermentation01:23

Microbial Fermentation

93
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
93
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

43
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
43
Lipid Catabolism01:25

Lipid Catabolism

109
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
109

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Primary challenges in the upcycling of genetically modified spent microbial biomass.

New biotechnology·2026
Same author

Exopolysaccharide biosynthesis and regulation in Leuconostoc mesenteroides influences water holding capacity of fermented plant-based milks.

International journal of food microbiology·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026
Same author

Engineering yeast for sustainable bioproduction of tryptophan-derived aromatic compounds.

FEMS yeast research·2026
Same author

Aerobic syngas conversion: opportunities, challenges, and solutions.

Current opinion in microbiology·2026
Same author

Extracellular Electron Transfer Enables Methanol Fermentation in Acetogen <i>Eubacterium limosum</i>.

Environmental science & technology·2026

相关实验视频

Updated: Jul 28, 2025

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

887

使用Eubacterium limosum对乙原形式变异潜力的表征.

Jamin C Wood1, R Axayacatl Gonzalez-Garcia2, Dara Daygon2,3

  • 1Australian Centre for Water and Environmental Biotechnology (ACWEB), The University of Queensland, QLD, Brisbane, 4072, Australia.

Applied microbiology and biotechnology
|June 5, 2023
PubMed
概括

利莫苏姆菌 (Eubacterium limosum) 使用酸盐来生长,但大部分的碳变成了二氧化碳,而不是酸盐. 这种形式的新陈代谢涉及蛋白质表达变化和酸盐积累,表明细胞应激.

关键词:
亚塞托基因的产物是一种基因.化学定位器 化学定位器这种细菌是Eubacterium limosum.格式 格式 格式代谢学 代谢学 代谢学蛋白质组学是指蛋白质组学.

更多相关视频

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.2K
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

28.0K

相关实验视频

Last Updated: Jul 28, 2025

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

887
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.2K
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

28.0K

科学领域:

  • 微生物学 微生物学
  • 代谢工程是代谢工程.
  • 生物技术是生物技术.

背景情况:

  • 格式是可再生电力的潜在能源载体.
  • 像Eubacterium limosum这样的酸性细菌可以使用酸盐来生长,吸引了生物技术的兴趣.
  • 在E. limosum中形成性新陈代谢尚不清楚,缺乏从连续培养中获得的系统级数据.

研究的目的:

  • 提供第一个稳定状态的欧米克数据集,用于在格式上生长的Eubacterium limosum.
  • 在系统层面上对E. limosum的格式营养代谢进行表征.
  • 为了研究代谢和蛋白质表达对格式利用的反应.

主要方法:

  • 连续培养E. limosum的种植.
  • 测量甲酸盐吸收率和二氧化碳生产率.
  • 差异性蛋白质表达分析.
  • 细胞内代谢学.细胞内代谢学.

主要成果:

  • 在0.4d-1的稀释率下观察到高特异性格式吸收率 (280 ± 56 mmol/gDCW/d).
  • 大多数消耗的形式碳被转化为二氧化碳 (150 ± 11 mmol/gDCW/d),而不是乙酸盐.
  • 观察到蛋白质对酸酶 (Pta) 和酸盐形式酶 (Pfl) 的上调,以及细胞内酸盐的积累,表明能量限制和压力.

结论:

  • 在E. limosum中,格式变性生长的特点是高格式吸收,但以CO2形式显著的碳损失.
  • 代谢调节发生在蛋白质表达水平,其中Pta和Pfl起着关键作用.
  • 酸盐可能会诱导细胞内压力,并可能导致E. limosum.中的生物膜形成.
  • 甲酸盐可以作为一种有价值的单碳基质,用于生产富含酸盐的化学物质.