使用Eubacterium limosum对乙原形式变异潜力的表征
Jamin C Wood1, R Axayacatl Gonzalez-Garcia2, Dara Daygon2,3
1Australian Centre for Water and Environmental Biotechnology (ACWEB), The University of Queensland, QLD, Brisbane, 4072, Australia.
Applied microbiology and biotechnology
|June 5, 2023
概括
利莫苏姆菌 (Eubacterium limosum) 使用酸盐来生长,但大部分的碳变成了二氧化碳,而不是酸盐. 这种形式的新陈代谢涉及蛋白质表达变化和酸盐积累,表明细胞应激.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 格式是可再生电力的潜在能源载体.
- 像Eubacterium limosum这样的酸性细菌可以使用酸盐来生长,吸引了生物技术的兴趣.
- 在E. limosum中形成性新陈代谢尚不清楚,缺乏从连续培养中获得的系统级数据.
研究的目的:
- 提供第一个稳定状态的欧米克数据集,用于在格式上生长的Eubacterium limosum.
- 在系统层面上对E. limosum的格式营养代谢进行表征.
- 为了研究代谢和蛋白质表达对格式利用的反应.
主要方法:
- 连续培养E. limosum的种植.
- 测量甲酸盐吸收率和二氧化碳生产率.
- 差异性蛋白质表达分析.
- 细胞内代谢学.细胞内代谢学.
主要成果:
- 在0.4d-1的稀释率下观察到高特异性格式吸收率 (280 ± 56 mmol/gDCW/d).
- 大多数消耗的形式碳被转化为二氧化碳 (150 ± 11 mmol/gDCW/d),而不是乙酸盐.
- 观察到蛋白质对酸酶 (Pta) 和酸盐形式酶 (Pfl) 的上调,以及细胞内酸盐的积累,表明能量限制和压力.
结论:
- 在E. limosum中,格式变性生长的特点是高格式吸收,但以CO2形式显著的碳损失.
- 代谢调节发生在蛋白质表达水平,其中Pta和Pfl起着关键作用.
- 酸盐可能会诱导细胞内压力,并可能导致E. limosum.中的生物膜形成.
- 甲酸盐可以作为一种有价值的单碳基质,用于生产富含酸盐的化学物质.
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