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在前列腺癌中用microRNAs关闭一些过度表达的基因,使用7mer-seed匹配模型

Arpita Purkayastha¹, Aparajita Roy¹, Stella Bharadaj²

¹Department of Biotechnology, Assam University, Silchar, Assam, 788011, India.

Journal of cancer research and clinical oncology

|June 5, 2023

概括

研究人员确定了针对前列腺癌过度表达的基因的微RNA. 这些microRNA显示出开发基于miRNA的新疗法以使促进癌症的基因沉默的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

前列腺癌的特点是特定基因的过度表达.
微RNAs (miRNAs) 在基因调节中起着至关重要的作用,并与各种癌症有关.
用miRNAs针对过度表达的基因提供了一个潜在的治疗策略.

研究的目的:

确定能够准前列腺癌过度表达的基因的特定人体微RNA.
为开发用于前列腺癌治疗的基于miRNA的新型治疗方法奠定基础.

主要方法:

使用7mer-m8模型分析miRNA-基因相互作用.
使用自由能量,GC含量,翻译效率和mRNA稳定性等参数评估miRNA结合效率.
采用BLAST2GO软件用于功能阐明前列腺癌相关基因.

主要成果:

发现多个miRNA针对过度表达的前列腺癌基因的编码序列.
在两个不同的位置,HPN基因被确定为miR-4279的目标.
目标地区的GC和GC3含量高于邻近地区,转化率低,可能是由于低于最佳的密码子.

结论:

成功识别了人类的miRNAs,这些miRNAs有潜力结合和沉默前列腺癌中14个高度过度表达的基因的编码序列.
这些发现为未来开发针对前列腺癌的基于miRNA的疗法提供了基础.

关键词:

7 其他种子匹配模型过度表达的基因前列腺癌是什么意思前列腺癌是什么意思治疗策略治疗策略翻译率翻译率翻译率预测miRNA的目标

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...