在性结肠炎中PD-1/PD-L1不平衡的重要性
Wei Shi1,2, Yu Zhang2, Chonghua Hao2
1Department of Clinical Laboratory, Shanxi Provincial People's Hospital, Taiyuan, China.
PeerJ
|June 5, 2023
概括
编程细胞死亡蛋白1 (PD-1) 和其连接体PD-L1的表达与性结肠炎 (UC) 疾病活性相关. 这些分子可以作为预测UC和监测疾病进展的生物标志物,表明UC患者的免疫功能障碍.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,其特点是免疫系统的调节失调.
- 编程细胞死亡蛋白1 (PD-1) 和其连接体PD-L1通路在免疫平衡和T细胞调节中起着至关重要的作用.
- 了解PD-1/PD-L1在UC病变发生中的作用对于开发向疗法至关重要.
研究的目的:
- 研究UC患者的粘膜组织和外周血液中PD-1和PD-L1的表达水平和临床意义.
- 为了确定PD-1/PD-L1表达和UC疾病活性之间的相关性.
- 探索PD-1/PD-L1作为UC预测和进展评估的生物标志物的潜力.
主要方法:
- 免疫组织化学用于评估80名UC患者的粘膜活检中的PD-1和PD-L1表达.
- 与酶相关的免疫吸收试验 (ELISA) 在血清中测量了可溶性PD-1和PD-L1 (sPD-1,sPD-L1).
- 流细胞计确定了外周血液中的膜结合PD-1 (mPD-1),Th1和Th17细胞.
主要成果:
- 粘膜膜自身中的PD-1和PD-L1表达与UC疾病活性增加.
- 与轻度UC患者和健康对照者相比,中度至重度UC患者的PD-1在T细胞 (PD-1/T,PD-1/CD4+T) 和sPD-L1的周围血液水平显著升高.
- sPD-L1水平与UC疾病严重程度和Th1/Th17细胞种群正相关.
结论:
- PD-1和PD-L1表达水平与UC疾病活性有显著的关联.
- PD-1/PD-L1信号的失衡有助于UC的免疫功能障碍.
- PD-1和PD-L1代表预测UC和监测疾病进展的潜在生物标志物,需要进一步研究治疗向.
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