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相关概念视频

What is Gene Expression?01:42

What is Gene Expression?

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Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
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Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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在为生物生产而编程的输入响应工程生物材料中的基因表达动态.

Widianti Sugianto1,2,3, Gokce Altin-Yavuzarslan2,4, Benjamin I Tickman1,2,3

  • 1Department of Chemical Engineering, University of Washington, Seattle, WA, 98195, United States.

Materials today. Bio
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概括
此摘要是机器生成的。

工程生物材料 (ELM) 维持基因表达数周,表现优于自由细胞. 这些微生物系统显示出对持续生物生产和先进应用的动态响应能力.

关键词:
克里斯普尔基因激活 (克里斯普尔a)在 ELM 生物制造上水凝是一种水凝.响应输入的工程生物材料 (ELM)可编程的生物生产.

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科学领域:

  • 合成生物学 合成生物学
  • 生物材料工程 生物材料工程
  • 微生物生物技术 微生物生物技术

背景情况:

  • 在水凝中使用微生物的工程生物材料 (ELM) 显示出生物生产的前景.
  • 在ELM中长期基因表达的动态仍然不太清楚.
  • 了解基因表达对于优化ELM功能至关重要.

研究的目的:

  • 研究微生物ELM中的长期基因表达动态.
  • 评估不同微生物和水凝矩阵对基因表达的影响.
  • 在ELM中展示持续和动态的生物生产能力.

主要方法:

  • 在水凝中封装的工程E. coli和S. cerevisiae (F127-BUM,BSA-PEGDA).
  • 直接测量基因表达和CRISPR转录激活 (CRISPRa).
  • 用化学诱导剂进行为期数周的生物生产循环.

主要成果:

  • 在ELM中诱导性遗传程序持续基因表达数周,比浮游生物细胞长得多.
  • 在重复的诱导周期后,ELM保留了高达97%的初始基因表达能力.
  • 证明了S. cerevisiae ELMs中的酶和颜料的多周,计划的生物生产切换.

结论:

  • 微生物ELM表现出强大的,长期的,动态响应的基因表达能力.
  • 这些发现表明长期基因表达是微生物ELM的一般性质.
  • 开发的方法可以为各种先进的应用程序定制ELM功能.