butanol作为乙醇和乙酸链延长过程中的主要产品
Aide Robles1,2,3, Skanda Vishnu Sundar1,2, Srivatsan Mohana Rangan1,2,3
1Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ, United States.
Frontiers in bioengineering and biotechnology
|June 5, 2023
概括
使用乙醇和酸盐的链延长可以产生价值的生物燃料butanol. 高度的乙醇和气体压力有利于butanol的生产,在最佳条件下实现1:1的butanol与丁酸盐比率.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 循环经济过程循环经济过程
- 微生物的新陈代谢
背景情况:
- 链延长是将有机原料转化为有价值的碳酸盐和酒精的关键生物工艺.
- 虽然短链和中链碳酸盐得到了很好的研究,但通过链延长生产布他诺的最佳生物反应器条件仍然基本上是未知的.
- 了解这些条件对于利用循环经济中的链条延长至关重要.
研究的目的:
- 为了调查乙醇和酸盐链延长过程中的butanol和butyrate生产.
- 为了确定乙醇度,总气体压力和的部分压力对butanol产量的影响.
- 评估微生物群落在不同生物反应器条件下的弹性.
主要方法:
- 使用半批量生物反应器 (0.16L血清瓶) 具有不同的乙醇度 (100-800 mM C) 和恒定的乙酸度 (50 mM C).
- 监控的总气压,的部分压力和产品度 (butanol,butyrate).
- 在实验期间操纵总气体压力,以评估其对产品比率的影响.
- 分析了微生物群体的组成,使用安普利康测序.
主要成果:
- butanol度与乙醇度增加至400mM C,与链条延长活动和气生产相关.
- 在 400 mM C 乙醇和 50 mM C 乙酸盐的使用下,可达到 114.96 ± 9.26 mM C (∼2.13 g L-1) 的最大布坦醇度.
- 在高总气体和的部分压力下,观察到1:1的丁醇与丁酸盐摩尔比率.
- 降低总压力使丁醇与丁酸盐的比率降低到1:2,并刺激的产生.
- 微生物群落主要由克洛斯特里克莱维里和阿利斯蒂普斯 (Alistipes) 占主导地位,对不断变化的生物反应器条件表现出了弹性.
结论:
- 乙醇和乙酸链的延长可以有效地产生butanol作为主要产品.
- butanol的生产取决于限制乙酸盐的可用性,并保持高的总气体和的部分压力.
- 这项研究强调了在循环经济框架内优化生物燃料生产链延长的潜力.
关键词:
克洛斯特里 (Clostridium kluyveri) 是一种有毒的植物.但是,醇和醇是不同的.黄油酸盐 黄油酸盐 是一种碳氧酸盐还原的方法的部分压力压力微生物链延长 微生物链延长总气体压力总的气体压力更多相关视频
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