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Updated: Jul 27, 2025

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在肺癌研究中对qRT-PCR强大的基因进行实验评估

Wei Gu1,2, Yubin Wang2, Ran Xu2

  • 1Department of Pathology, First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China.

Frontiers in oncology
|June 5, 2023
PubMed
概括

找到稳定的参考基因对于使用定量实时PCR (qRT-PCR) 进行精确的肺癌研究至关重要. 这项研究确定了验证的稳定基因,如CIAO1,CNOT4和SNW1,用于肺癌研究中可靠的qRT-PCR分析.

关键词:
缺氧 缺氧是指缺氧的情况.肺癌是一种肺癌.qRT-PCR 是一个很好的方法.基因参考基因是指基因的参考基因.血清剥夺是一种血清剥夺.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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