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相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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相关实验视频

Updated: Jul 27, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Three-dimensional Optical-resolution Photoacoustic Microscopy

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超光谱三维吸收成像使用快照光学断层扫描.

Cory Juntunen1, Andrew R Abramczyk1, Isabel M Woller2

  • 1College of Engineering and Applied Science, University of Wisconsin, Milwaukee, Wisconsin 53211, USA.

Physical review applied
|June 5, 2023
PubMed
概括

我们开发了一种新的4D高光谱成像系统,结合了快照投影光学断层扫描 (SPOT) 和里埃变换谱学 (FTS). 这种高通量系统可以对微观样品进行体积化学指纹,克服传统3D HSI的局限性.

科学领域:

  • 光学成像技术的成像
  • 频谱学是一种光谱学.
  • 生物医学光学 生物医学光学

背景情况:

  • 超光谱成像 (HSI) 在每个像素上提供化学信息,但通常是二维的.
  • 3D HSI提供体积化学数据,但其成像吞吐量较低.
  • 现有的方法需要连续扫描波长和样本旋转.

研究的目的:

  • 介绍一款用于高通量化学成像的新型4D光学系统.
  • 结合快照投影光学断层扫描 (SPOT) 和富里埃变换谱学 (FTS) 进行体积光谱数据采集.
  • 为了克服传统3D HSI的吞吐量限制.

主要方法:

  • 开发了一个集成SPOT的4D HSI系统,用于同时获得多角度投影.
  • 集成的FTS用于从干扰图收集高分辨率光谱数据.
  • 该系统在一次快照采集中捕获3D结构和1D频谱.

主要成果:

  • 成功成像染色聚烯微珠的体积吸收率.
  • 在4D中展示了氧化和脱氧红细胞 (RBC) 的成像.
  • 实现了与传统的2D HSI系统相提并论的高通量数据采集.

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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相关实验视频

Last Updated: Jul 27, 2025

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08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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结论:

  • 展示的4D高光谱SPOT系统能够快速进行体积化学成像.
  • 这项技术为分析复杂的微观标本提供了强大的工具.
  • 在高通量化学分析和生物样本表征方面的潜在应用.