从中提取的外聚糖 (EPS) 的抗菌和抗生物膜活性之间的比较
S M Abed1, H Jabar Jasim2, M Mijbas Mohammed Alomari2
1Department of Medical Laboratories, College of Medical and Health Techniques, Sawa University, Samawah, Iraq.
Archives of Razi Institute
|June 5, 2023
概括
来自Lactobacillus reuteri和Streptococcus mitis的细菌外聚糖 (EPS) 显示出对口腔细菌具有有前途的抗菌和抗菌膜活性. 在EPS的结构差异影响他们的有效性,与L. reuteriEPS一般是更强大的.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 抗生素耐药性增加需要使用替代抗菌策略.
- 细菌外聚糖 (EPS) 正在研究它们的治疗潜力.
- 以前的研究表明,不同细菌物种对EPS的结构变化有所不同.
研究的目的:
- 评估来自Lactobacillus reuteri和Streptococcus mitis的EPS的抗菌和抗菌膜疗效.
- 为了比较来自这两种细菌来源的EPS的活性与各种口腔病原体.
- 调查EPS结构与其抗微生物/抗物特性之间的相关性.
主要方法:
- 通过使用乙醇沉,从Lactobacillus reuteri和Streptococcus mitis中提取了EPS.
- 在不同度 (100-300毫克/毫升) 中评估了抗菌活性对金黄色葡萄球菌,人类葡萄球菌,宝曼尼酸菌,罗尔特鸟类菌和Streptococcus thoraltensis.
- 通过将EPS治疗组与对照组进行比较,评估了抗生物膜活性.
主要成果:
- 来自L. reuteri的EPS含有85%的碳水化合物,而来自S. mitis的EPS含有80%的碳水化合物.
- 从两种EPS类型的200mg/ml度开始,观察到显著的抗菌作用.
- 来自L. reuteri的EPS与S. mitis EPS相比,对大多数测试的口腔细菌表现出优异的抗菌膜活性,其中S. thoraltensis的例外值得注意.
结论:
- 细菌外聚糖的结构特征显著影响其抗菌和抗菌膜的能力.
- 来自L. reuteri的EPS与S. mitis的EPS相比,具有更大的潜力作为一种天然抗微生物剂来对抗口腔细菌和生物膜形成.
- 对EPS结构-活性关系的进一步研究可能会导致新的治疗应用.
关键词:
抗菌的 抗菌的 抗菌的抗生物膜是一种抗生物膜.在EPS的EPS系统中.牛乳杆菌reuteri 牛乳杆菌reuteri 是一个杆菌甲虫菌 (Streptococcus Mitis) 是一个有毒的菌株.更多相关视频
08:20Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
6.4K
07:16Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
Published on: September 20, 2024
1.1K
相关概念视频
01:28Antimicrobial Effectiveness
98
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
98
01:29Biofilms
61
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
61
01:18Surface Membrane Barriers
1.2K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.2K