基因的基因检测编码了一些酶中的基因
S Saleh Ahmed1, I Ghanim Abdulwahhab1, H Salman Alagely2
1College of Education, University of Tikrit, Tikrit, Iraq.
Archives of Razi Institute
|June 5, 2023
概括
聚合酶连锁反应 (PCR) 是一个比显微镜更敏感的诊断工具 Entamoeba histolytica,识别 21.57% 的儿童感染腹. 这种原生虫寄生虫对男性的影响比女性更大,在1岁以下的儿童中感染率最高.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 分子诊断学 分子诊断
- 儿童传染病 儿童传染病
背景情况:
- 昆虫菌 (Entamoeba histolytica) 是一种重要的原生虫肠道寄生虫,导致广泛的感染和死亡率.
- 准确而敏感的诊断方法对于有效的疾病控制至关重要.
研究的目的:
- 为了比较聚合酶链反应 (PCR) 的诊断疗效与儿童患者的Entamoeba histolytica传统染色技术.
- 确定患有腹的儿童中Entamoeba histolytica感染的流行率和流行病学特征.
主要方法:
- 在200个便样本上进行了显微镜检查和PCR分析,这些样本来自腹的儿童.
- 用PCR检测特定的Entamoeba histolytica基因,包括氨酸蛋白酶五和脂酶.
主要成果:
- 通过PCR检测发现了11例 Entamoeba histolytica 阳性病例,占阳性诊断的21.57%,而显微镜诊断的病例为51.
- 男性的感染率 (54.9%) 比女性 (45.1%) 高.
- 在1岁以下的儿童中,患病率最高 (43.1%),其次是1-2岁的儿童 (31.4%).
结论:
- 与显微镜检查相比,PCR在检测Entamoeba histolytica方面提供了更高的灵敏度.
- 该研究强调了分子方法在诊断寄生虫感染方面的重要性,并提供了有关儿科Entamoeba histolytica感染的流行病学数据.
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