病理和分子检测的病理和分子检测
Z Waheed Khudiar Khareeb1, Z Ismail Ibrahim2, F Ali Abdullah3
1Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Archives of Razi Institute
|June 5, 2023
概括
在羊群中,Mycoplasma ovipneumonea会引起非典型的肺炎. 这项研究在伊拉克绵羊中分离和鉴定了M. ovipneumonea,证实了其流行率和病理影响.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物病理学 动物病理学
背景情况:
- 菌菌 (Mycoplasma ovipneumonea) 是羊和山羊非典型肺炎的重要原因.
- 它经常从肺炎病例中分离出来,但也可以存在于健康动物的呼吸道中.
研究的目的:
- 为了隔离,识别,并进行病理学检查羊中的Mycoplasma ovipneumonea.
- 在伊拉克巴斯拉,调查M. ovipneumonea在表现出呼吸系统症状的绵羊中的流行情况.
主要方法:
- 在PPLO agar上从鼻腔,气管和支气管抽样中分离的细菌.
- 用PCR分析向16SrRNA基因的DNA提取.
- 肺组织组织病理学检查,以评估肺部的变化.
主要成果:
- 菌体隔离物在PPLO agar上表现出特有的"炸蛋"殖民地形态.
- 通过PCR检测证实了Mycoplasma sp. 的存在. 通过16S rRNA基因.
- 病理发现包括呼吸道堵塞,,出血斑点,炎症排泄物,以及急性纤维性-补充性支气管间歇性肺炎.
结论:
- 菌性肺炎 (Mycoplasma ovipneumoniae) 是伊拉克巴斯拉省绵羊中普遍存在的呼吸道传染病.
- 该研究证实,在受影响的绵羊中,经常出现细菌隔离和相关的肺病理变化.
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