在临床研究中研究一些抗性基因
E Abed Gumar1, A Salim Hamzah2, W Fadhil Hamad1
1Department of Medical Laboratory Techniques, College of Health and Medical Technology, Middle Technical University, Baghdad, Iraq.
这项研究确定了来自伊拉克的多药耐药Proteus mirabilis中的抗生素耐药基因. 伊拉克菌株与全球菌株相比显示出遗传变异,IntI1和blaTEM基因非常普遍.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 传染性疾病 传染性疾病
背景情况:
- 奇迹菌是一种机会性细菌,导致临床疾病.
- 奇迹菌中的多药耐药性 (MDR) 构成了严重的公共卫生威胁.
研究的目的:
- 在伊拉克的MDR P. mirabilis临床分离物中检测抗生素耐药性基因 (blaTEM, aac(6') -Ib, qnrA, IntI2, IntI1).
- 研究这些抵抗基因在伊拉克和全球菌株之间的遗传学关系.
主要方法:
- 使用VITEK-2 Compact系统从500个临床样本中识别了95个P. mirabilis分离物.
- 对12种抗生素进行抗生素敏感性测试.
- 使用NCBI数据库进行基因测序和遗传学分析.
主要成果:
- 耐药基因的高患病率:IntI1 (100%),blaTEM (90%),aac(6') -Ib (80%),IntI2 (80%). 耐药基因的高患病率:IntI1 (100%),blaTEM (90%),aac(6') -Ib (80%),IntI2 (80%). 耐药基因的高患病率:IntI1 (100%),blaTEM (90%),aac(6') -Ib (80%),IntI2 (80%). 耐药基因的高患病率:IntI1 (100%),blaTEM (90%),aac(6') -Ib (80%),IntI2 (80%). 耐药基因的高患病率:IntI1 (100%),blaTEM (90%),aac(6') -Ib (80%).
- 遗传学分析显示,与全球菌株相比,伊拉克分离品中的遗传变异.
- 在NCBI注册的序列基因与加入号码.
结论:
- 伊拉克的MDR P. mirabilis含有关键的抗生素耐药性基因.
- 抗性基因的遗传变异可能会影响治疗策略.
- 基因组监测对于跟踪抗生素耐药性模式至关重要.
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