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重组k-carrageenase和菌体T7从释放的盐分提取

Da Chen¹, Yue-Sheng Dong¹, Yong-Ming Bao¹

¹School of Bioengineering Dalian University of Technology Dalian Liaoning PR China.

Engineering in life sciences

|June 5, 2023

概括

这项研究介绍了菌体溶解与盐分提取 (SOE) 结合,作为基因工程的有效方法. 这种生物工艺为工业应用中细胞破坏和产品回收提供了具有成本效益的替代方案.

科学领域:

生物技术是生物技术.
基因工程是一种基因工程.
微生物过程微生物过程

背景情况:

传统的细胞破坏方法昂贵且低效,阻碍了工业基因工程.
开发新的生物过程对于克服这些局限性至关重要.

研究的目的:

评估一种结合菌素溶解和盐分提取 (SOE) 的新生物工艺,用于细胞破坏和产品恢复.
研究T7菌素溶解对基因工程大肠杆菌的疗效,以及菌素和细胞内产品的随后分离.

主要方法:

在各种多重感染 (MOI) 时研究了大肠杆菌的T7菌素溶解.
采用盐分提取 (SOE) 使用硫酸和乙烯酸乙烯分离菌体和释放的酶.
优化国有企业条件,以实现高效的分离和产品回收.

主要成果:

在90分钟内,T7菌体在MOI=1时实现了99.9%的宿主细胞溶解,释放了90%以上的酶.
SOE成功地将T7菌素的87.1%分离到中间阶段,并将k-carrageenase的71.2%分离到底部阶段.
硫酸沉产生了从底部阶段 κ-carrageenase 的 40.1%.

结论:

菌体溶解为细胞破坏提供了温和的条件和低成本.
SOE提供了高效的菌体和细胞内产品的分离.
菌体溶解与SOE相结合,在基因工程中为工业细胞破坏和产品恢复提供了一个有希望的替代方案.

关键词:

美国大肠杆菌 (E. coli).细胞破坏细胞破坏菌体菌体是一种菌体.化 - 提取取出的盐.κ-胡卜素酶的使用方法

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