分子鉴定,抗微生物耐药性和病毒性基因分析的分子鉴定
Md Abul Fazal1, Eaftekhar Ahmed Rana1, Sazeda Akter2
1Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Khulsi, Chattogram-4225, Bangladesh.
Veterinary and animal science
|June 5, 2023
概括
这项研究揭示了导致孟加拉乳牛亚临床乳腺炎的葡萄球菌种的高发病率,发现了显著的抗微生物耐药性和多药性耐药性. 关键的风险因素包括年龄较大,早期哺乳期和先前的乳腺炎史.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物科学动物科学
- 食品安全 食品安全
背景情况:
- 亚临床乳腺炎 (SCM) 是乳制品生产中一个重要的经济问题.
- 葡萄球菌物种是常见的乳腺炎病原体,由于抗菌素耐药性而带来挑战.
- 了解病原体的多样性和耐药性对于孟加拉国有效的群体管理至关重要.
研究的目的:
- 调查孟加拉国乳牛群中导致SCM的葡萄球菌种的多样性.
- 分析这些葡萄球菌种的抗菌素耐药性模式.
- 为了确定与SCM和耐甲素的葡萄球菌 spp. 相关的风险因素. 旅行车. 旅行车. 旅行车.
主要方法:
- 来自30个农场的284头奶牛的牛奶样本使用加利福尼亚乳腺炎测试对SCM进行了查.
- 传统的微生物隔离和PCR用于物种鉴定和耐药性/病毒性基因的检测.
- 用多变量逻辑回归分析来确定风险因素.
主要成果:
- 葡萄球菌染色体 (65.7%) 是最常见的物种,其次是S. epidermidis,S. haemolyticus,S. aureus和S. sciuri.
- 对皮素,阿莫西西林/克拉夫兰酸和Cefepime在不同物种中观察到高耐药率.
- 多种药物耐药性普遍存在,在各种物种中检测到mecA,pvl和tst基因. 年龄较大,早期哺乳期和状况坚固被确定为SCM的危险因素.
结论:
- 葡萄球菌种,特别是S. chromogenes,是孟加拉国SCM的主要原因,表现出有关抗菌素耐药性的情况.
- 包括mecA在内的毒性和耐药性基因的存在凸显了严重感染和传播的潜力.
- 抗微生物药物的使用和先前的乳腺炎病史是甲基西林耐药性斯塔菲洛科克菌种的重要危险因素. 运输,需要改善群体管理和抗菌药物管理.
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