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用于检测SARS-COV-2的核囊蛋白结合DNA体
Charles P Neff1, Mile Cikara2, Brian J Geiss3
1Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Current research in biotechnology
|June 5, 2023
概括
研究人员开发了DNA体作为SARS-CoV-2的敏感检测试剂,在抗原测试中提供了抗体的潜在替代品. 这些体对检测当前和新兴的变种,如Omicron,显示出前途.
科学领域:
- 生物技术是生物技术.
- 分子诊断学 分子诊断
- 病毒学 病毒学
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 仍然是一个全球健康挑战.
- 目前的诊断方法,如RT-PCR是昂贵的和实验室依赖;抗原测试缺乏最佳的灵敏度和特异性,特别是在新变种.
- 现有诊断的局限性需要开发快速,敏感和特定的检测工具.
研究的目的:
- 开发针对SARS-CoV-2核体蛋白的DNA吸收体,作为新型检测试剂.
- 评估这些体在检测SARS-CoV-2及其变体中的性能.
- 为了探索体重选,以改善新出现的SARS-CoV-2菌株的检测.
主要方法:
- 对抗SARS-CoV-2核体蛋白的DNA体的选择.
- 在类似ELISA的格式中,使用aptamer作为检测试剂进行测试开发.
- 测试对无活化SARS-CoV-2 (武汉阿尔法菌株) 和其他人类冠状病毒 (HCoV) 的aptamer敏感性和特异性.
- 对抗SARS-CoV-2 Omicron变异的阿普坦体的重新选择.
主要成果:
- 开发出能够检测到150 pg/mL的SARS-CoV-2核体蛋白质的DNA体.
- 已检测到不到15万份无活化的SARS-CoV-2武汉阿尔法菌株,对HCoVs的交叉反应最小.
- 鉴定了两种重新选择的体酶序列,与原始体酶相比,对Omicron变异的信号增加了两倍以上.
结论:
- DNA 体是SARS-CoV-2的有效和敏感检测试剂,在抗原检测试验中提供了抗体的可行替代品.
- 开发的aptamers显示出用于快速诊断测试的潜力,补充或取代现有技术.
- 阿普塔默重新选择是一种经过验证的策略,用于调整诊断工具以适应新出现的病毒菌株,如SARS-CoV-2 Omicron.
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