多价值Bicc1-ANKS3-ANKS6蛋白网络中结构域之间的对抗性相互作用控制了目标mRNAs的相位过渡
Benjamin Rothé1, Simon Fortier1, Céline Gagnieux1
1Ecole Polytechnique Fédérale de Lausanne (EPFL) SV ISREC, Station 19, 1015 Lausanne, Switzerland.
iScience
|June 5, 2023
概括
研究人员发现了蛋白质Bicaudal-C (Bicc1) 如何形成控制信使RNA (mRNA) 液体到凝转换的网状结构. ANKS3和ANKS6蛋白调节这个过程,影响mRNA固定.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 异常的核糖蛋白阶段过渡与许多疾病有关.
- 已知病蛋白ANKS3和ANKS6对RNA结合蛋白Bicaudal-C (Bicc1) 的调节,但它们对基mRNA的影响尚不清楚.
研究的目的:
- 研究Bicc1聚合及其与ANKS3和ANKS6的相互作用如何影响目标mRNA.
- 阐明Bicc1介导的mRNA固定和相变的机制.
主要方法:
- 使用其SAM和KH域研究了Bicc1的聚合.
- 研究了ANKS3和ANKS6在Bicc1颗粒分散和mRNA释放/招募中的作用.
- 分析了RNA独立的Bicc1聚合.
主要成果:
- 通过其SAM域,Bicc1形成了头到尾的聚合物,通过其SAM域创建了与KH域的蛋白质网格.
- 这种网格介绍了客户端转录的液态到凝的转换.
- ANKS3分散Bicc1颗粒,释放结合的mRNA,而ANKS6的联合招募恢复了凝结和核粒子组装.
结论:
- 由ANKS3和ANKS6调节的Bicc1聚合,为控制mRNA固定提供了一个新的机制.
- 这个过程将可逆的mRNA固定化与蛋白质相转换之间的转稳状态相结合.
- 了解这种机制对于与异常的核糖蛋白阶段过渡相关的疾病至关重要.
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