抗炎化合物减少马类疹病毒1型的复制和细胞间的传播
Jeanette B Black1, Arthur R Frampton1
1Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, NC, United States.
Frontiers in veterinary science
|June 5, 2023
概括
探索了马类疹病毒髓脑病变 (EHM) 治疗方法. 弗鲁尼克辛 (FM) 和AG490显著降低了EHV-1病毒的产量和马细胞中的细胞间传播.
科学领域:
- 兽医病毒学 兽医病毒学
- 马类传染病 马类传染病
- 分子病毒学分子病毒学
背景情况:
- 马类疹病毒1型 (EHV-1) 导致马的重大疾病,包括神经疾病,如马类疹病毒髓脑病变 (EHM).
- 了解EHV-1病原和病毒与宿主相互作用对于开发有效的EHM治疗方法至关重要.
- 目前对EHM的治疗策略有限,需要探索新的治疗点.
研究的目的:
- 研究各种抗炎药物和抑制剂在减少EHV-1复制和细胞间传播方面的疗效.
- 为了确定潜在的治疗药物,可以减轻与EHV-1感染相关的病毒载量和传播.
- 评估这些药物对神经和非神经性EHV-1菌株的影响.
主要方法:
- 对四种非类固醇抗炎药物 (NSAIDs),德克萨米他,Rho-kinase (ROCK) 抑制剂和JAK/STAT抑制剂 (AG490) 的抗病毒活性进行查.
- 在受感染的内皮和上皮细胞系中量化EHV-1病毒的产量,这些细胞系用所选化合物治疗.
- 评估药物抑制EHV-1细胞间传播的能力.
主要成果:
- 无 NSAID 药物 flunixin meglumine (FM) 和 JAK/STAT 抑制剂 AG490 显示,它们在内皮和上皮细胞中显著降低了 EHV-1 病毒产量.
- 这种抑制作用在两个神经学和两个非神经学EHV-1菌株中是一致的.
- AG490和FM都显著抑制了EHV-1从感染细胞向未感染细胞的横向传播.
结论:
- 弗鲁尼克辛梅格卢明 (FM) 和AG490通过减少病毒复制和传播,显示出它们作为针对EHV-1的治疗剂的前景.
- 这些发现突出了针对炎症途径和JAK/STAT信号的潜力,用于EHV-1治疗.
- 需要进行进一步的研究,以探索FM和AG490在治疗EHV-1感染和预防马中的EHM方面的临床应用.
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