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Updated: Jul 27, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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一个G-四重体过氧酶DNA酶的定向演化和在蛋白质DNA酶-Aptamer近距离标记中的应用

Soubhagya K Bhuyan1,2, Lin Wang1,2, Chandra Jinata1,2

  • 1School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Journal of the American Chemical Society
|June 5, 2023
PubMed
概括

研究人员使用定向进化开发了一种高效的过氧化酶DNA酶 (mSBDZ-X-3). 这种新的DNA酶加速了基于近距离的空间蛋白质的标记,使得癌细胞中的蛋白质能够快速识别.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor09:33

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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