Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

60
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
60

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

TGM2 drives microglial senescence by inhibiting autophagy via the PI3K/AKT/mTORC1 pathway.

Free radical biology & medicine·2026
Same author

Zero-shot de novo peptide sequencing with open posttranslational modification discovery.

Nature biotechnology·2026
Same author

Author Correction: Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.

Nature cell biology·2026
Same author

Tubeimoside Ⅰ targets sodium/potassium ATPase alpha 1 subunit to selectively eliminate senescent cells and alleviate aging-associated abnormalities.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Nanobody-Mediated c-MYC Degradation Inhibits Tumor Cell Progression.

MedComm·2026
Same author

EIF4H and YBX1 are essential host factors for hepatitis E virus replication and pathogenesis.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Jul 27, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

13.3K

PRMT5/WDR77复合体限制了E型肝炎病毒的复制.

Xiaohui Ju1, Yanying Yu1, Wenlin Ren1

  • 1School of Medicine, Tsinghua University, Beijing, China.

PLoS pathogens
|June 5, 2023
PubMed
概括
此摘要是机器生成的。

研究人员确定了蛋白质氨酸甲基转移酶5 (PRMT5) 和WDR77作为抑制肝炎E病毒 (HEV) 复制的关键宿主因素. 这一发现为治疗HEV感染提供了潜在的治疗点.

更多相关视频

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

9.8K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

11.7K

相关实验视频

Last Updated: Jul 27, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

13.3K
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

9.8K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

11.7K

科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 宿主-病原体相互作用

背景情况:

  • 肝炎E病毒 (HEV) 是全球急性肝炎的重要原因之一.
  • 控制HEV复制的精确机制和涉及的宿主因素在很大程度上是未知的.

研究的目的:

  • 为了确定调节HEV复制的宿主因素.
  • 阐明已识别的因素在病毒生命周期中的作用.

主要方法:

  • 使用HEV ORF1转补和复制细胞培养系统.
  • 在细胞培养中使用稳定同位素标记与氨基酸 (SILAC) 结合质谱 (MS) 用于宿主因子识别.

主要成果:

  • 确定了与HEV ORF1蛋白相关的众多宿主因素,涉及病毒RNA复制.
  • 蛋白质氨酸甲基转移酶5 (PRMT5) /WDR77复合物在两种模型中都成为了最受欢迎的.
  • 证明PRMT5/WDR77通过ORF1在R458的甲基化特别抑制HEV复制,而不是HCV或SARS-CoV-2,从而损害复制酶活性.

结论:

  • PRMT5/WDR77复合体作为对HEV的关键宿主限制因素.
  • 了解这种相互作用为病毒病原体提供了洞察力,并建议PRMT5/WDR77作为HEV干预的潜在治疗点.