布鲁塞拉堕胎抗原omp25疫苗:基于乳球菌乳酸菌的开发和向
Somaye Tirbakhsh Gouran1, Abbas Doosti2, Mohammad Saeid Jami1,3
1Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Veterinary medicine and science
|June 5, 2023
概括
这项研究开发了一种使用乳球菌的口服疫苗,以传递Brucella abortus omp25抗原,显示出有前途的免疫反应和小鼠的炎症减少,为brucellosis管理提供了一种新的策略.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 疫苗开发 疫苗开发
背景情况:
- 布鲁塞拉感染主要发生在粘膜上,需要有效的粘膜疫苗接种策略.
- 开发口服疫苗对于管理广泛传播的动物传播疾病 - - 兽病至关重要.
研究的目的:
- 评估使用乳球菌乳腺菌的复合口服抗原输送系统对Brucella abortus omp25抗原的疗效.
- 在小鼠模型中评估这种新型口服疫苗的免疫性和保护潜力.
主要方法:
- 乳球菌被改造为表达Brucella abortus omp25抗原.
- 鼠被用工程L. lactis口服免疫,并分析了免疫反应 (IgG1,sIgA,细胞因子).
- 通过挑战接种疫苗的小鼠用Brucella abortus来评估保护效果.
主要成果:
- 使用L. lactis表达Omp25的口服疫苗诱导了显著的Omp25特异性IgG1和sIgA抗体反应.
- 接种疫苗导致关键细胞因子 (IFN-γ,TNF-α,IL-4,IL-10) 的水平增加,并减少了脏和肺组织的炎症损伤.
- 用L. lactis系统接种疫苗的小鼠表现出对布鲁塞拉流产挑战的保护.
结论:
- 乳球菌 (Lactococcus lactis) 作为Brucella abortus omp25抗原的有效和安全的口服输送系统.
- 这种新的方法提供了一种具有成本效益,可持续性和简单的方法,用于开发新型针对牛病的疫苗.
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