过氧化的氧化改变了蜜蜂花粉过敏原蛋白的结构性质和过敏性
Enning Zhou1, Weiwei Wang2, Xiaofeng Xue1
1State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
Food chemistry
|June 5, 2023
概括
使用过氧化氧化蛋白质的氧化有效地降低了蜜蜂花粉特征的Bra c p的过敏性. 这种方法改变了蛋白质结构并降低了IgE结合亲和力,为减轻食物过敏提供了一种新的方法.
科学领域:
- 食品科学 食品科学 食品科学
- 过敏研究研究 过敏研究
- 蛋白质化学 蛋白质化学
背景情况:
- 蜜蜂花粉是一种常见的食品成分,但可以引起严重的食物过敏.
- 像蜜蜂花粉中的BraCP这样的profilins是已知的泛过敏原,负责交叉反应性过敏反应.
- 减少食品成分的过敏性对于食品安全和公共健康至关重要.
研究的目的:
- 为了研究蛋白质氧化的潜力,使用过氧化来降低Bra c p. 的过敏性.
- 描述氧化后Bra c p的结构和功能变化.
主要方法:
- 用过氧化氧化Bra c p的蛋白质氧化.
- 离子移动性质谱法用于分析结构变化和聚合.
- 对二次结构变化的分析 (α-螺旋到β-片).
- 免疫测试以评估IgE结合亲和力.
主要成果:
- 氧化诱导的BraCP蛋白的聚合.
- 在Cys98残留物中发生了不可逆转的硫化,形成了稳定的囊衍生物.
- 观察到从α螺旋转向β螺旋转的显著转变,掩盖了抗原表位.
- 在体外测试显示,氧化Bra c p. 的IgE结合亲和力显著下降.
结论:
- 蛋白质氧化是一种可行的策略,可以降低蜜蜂花粉蛋白 (Bra c p) 的过敏性.
- 结构修改,包括聚合和改变的二次结构,有助于降低过敏性.
- 这项研究提出了蛋白质氧化的新应用,以减轻与蛋白质相关的食物过敏.
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