通过ATGL介导的脂质消化平衡了病原体感染的Apostichopus japonicus coelomocytes中胆固醇诱导的炎症
Meixiang You1, Lianlian Sun1, Chenghua Li2
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, PR China.
Fish & shellfish immunology
|June 5, 2023
概括
在海中,脂多糖 (LPS) 刺激会改变胆固醇代谢. 通过AJATGL介导的脂质会分解胆固醇,减少炎症并维持免疫平衡.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 细胞代谢的细胞代谢.
背景情况:
- 胆固醇代谢对哺乳动物巨细胞炎症功能至关重要.
- 胆固醇动态在水生动物炎症中的作用尚不清楚.
研究的目的:
- 在Apostichopus japonicus coelomocytes中调查胆固醇代谢对LPS刺激的反应.
- 在调节与胆固醇相关的炎症中阐明脂质的机制.
主要方法:
- 脂聚糖 (LPS) 刺激胆固醇细胞.
- 对细胞内胆固醇水平,胆固醇 (CE) 和脂质滴 (LD) 的分析.
- 评估了LDs与溶酶体的同位化以及关键蛋白质的表达 (AjIL-17,AjLC3,Ajp62,AJABCA1,AJATGL).
- 研究了AJATGL在通过过度表达诱导脂质的作用.
主要成果:
- 在早期,LPS刺激增加了细胞内胆固醇和AjIL-17的表达.
- 过多的胆固醇被储存在LDs中,随后在以后的时间点增加LD-lysosome同位和脂质标记物 (AjLC3,AjABCA1).
- AjATGL对于脂质诱导是必不可少的;AjatGL的过度表达减少了胆固醇诱导的AjIL-17表达.
结论:
- 胆固醇代谢在A. japonicus的胆固醇细胞中动态响应LPS刺激.
- 通过AJATGL介导的脂质在胆固醇的水解中起着关键作用.
- 脂ophagy平衡胆固醇诱导的炎症在海胆固醇细胞.
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