各种脂质在两性多佐林上,以达到有效的细胞内输送
L Simon1, M De Taddeo1, A Coeurvolan1
1ICGM, Univ. Montpellier, CNRS, ENSCM, Montpellier, France.
International journal of pharmaceutics
|June 5, 2023
概括
与PEGylated脂质体相比,利波聚可萨氨酸的细胞内传递优越. 这一突破为药物输送中有效的纳米配方开发提供了一个有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物运输 药物运输 药物运输
背景情况:
- 聚乙烯甘醇 (PEG) 由于其隐形特性,在纳米配方中广泛使用.
- 然而,PEGylation可以阻碍细胞吸收,限制细胞内药物递送.
- 开发用于增强细胞内化的PEG替代品至关重要.
研究的目的:
- 评价脂聚氧沙素 (LPO) 作为PEG的替代品,用于细胞内输送.
- 为了比较LPOs与不同脂质链的物理化学性质,细胞活力和内化能力.
- 评估基于LPO的脂质体与基于PEG的脂质体的体内细胞内传递效率.
主要方法:
- 用各种脂质链合成两性多-2-甲基-2-氧沙) 的合成.
- 物理化学性质的表征 (大小,泽塔潜力).
- 在体外评估细胞活力和细胞内化.
- 用光探针装载的脂质体的配方,用于比较的输送研究.
主要成果:
- 线性和LPOs显示了最高的细胞内化和良好的细胞活力.
- 基于LPO和PEG的脂质体表现出相似的大小,药物负载和细胞活力.
- 与基于PEG的脂质体相比,基于LPO的脂质体在细胞内传递方面有30倍的改善.
结论:
- 脂聚素是一种可行的,有效的替代PEG用于细胞内输送.
- LPOs通过内细胞分裂促进细胞进入,克服了PEGylated系统的局限性.
- 对于开发用于静脉注射的先进纳米配方,LPOs具有显著的前景.
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