ACL和HAT1形成一个核模块,以乙化素H4K5并促进细胞增殖
Qiutao Xu1, Yaping Yue1, Biao Liu1
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070, Wuhan, China.
Nature communications
|June 5, 2023
概括
这项研究揭示了ATP-酸酶 (ACL) 亚单元A2 (ACLA2) 和Histone AcetylTransferase1 (HAT1) 如何在米中形成一个模块. 该模块在局部产生乙-CoA,用于基因素乙化,这对于细胞分裂至关重要.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 生物化学 生物化学
背景情况:
- 基因乙化调节基因表达,对细胞分裂至关重要.
- 酸酸酶 (ACL) 是 histone 乙化中的乙-CoA 的主要来源.
- 对于核乙化局部乙-CoA生产的精确机制在很大程度上是未知的.
研究的目的:
- 调查ACL亚单元A2 (ACLA2) 在核乙-CoA生产和基因素乙化中的作用.
- 为了阐明米中的ACLA2和Histone AcetylTransferase1 (HAT1) 之间的相互作用.
- 了解HAT1-ACLA2模块在细胞分裂中的功能意义.
主要方法:
- 在核凝聚物中ACLA2的亚细胞局部化.
- 对甲基-CoA积累和素乙化水平在米突变物中的分析.
- 对ACLA2和HAT1 (HAG704) 基因进行基因分析.
- 基因表达造型和细胞周期分析.
主要成果:
- ACLA2局限于核凝聚物,对于核乙-CoA的积累和特定的基因素乙化至关重要.
- 米HAT1乙化基因组H4K5和H4K16,而H4K5的乙化取决于ACLA2.
- 在ACLA2和HAT1突变损害内细胞分裂,减少H4K5乙化,改变基因表达,并导致S阶段停滞.
结论:
- HAT1-ACLA2模块在特定的基因组区域建立了局部乙-CoA生产以进行选择性素乙化.
- 这种机制将能量代谢与植物的细胞分裂控制联系起来.
- 通过表观遗传修饰,ACLA2和HAT1在调节细胞增殖方面发挥着至关重要的作用.
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