Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Sanger Sequencing01:57

Sanger Sequencing

755.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.3K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.3K
Next-generation Sequencing03:00

Next-generation Sequencing

91.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.7K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Real-world efficacy and safety of transarterial chemoembolization plus sintilimab and bevacizumab biosimilar for intermediate-advanced hepatocellular carcinoma: a propensity score matching study.

Frontiers in immunology·2026
Same author

Single-cell profiling reveals distinct populations of tumor-associated macrophages and metastatic tumor cells in breast cancer brain metastasis.

Cell death & disease·2026
Same author

Insights into karyotype evolution and flower color variation from the genome assembly of wallflower (Erysimum cheiri).

Plant physiology·2026
Same author

Impact of Clonal Hematopoiesis of Indeterminate Potential on Treatment Response and Tumor Microenvironment in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy.

Journal of mammary gland biology and neoplasia·2026
Same author

TACE and tyrosine kinase inhibitors with or without PD-1 inhibitors as first-line therapy for intermediate-advanced HCC: a multicenter real-world cohort study.

Hepatology international·2026
Same author

Challenges of Real-World Utilization of Recommendations From ClinGen ENIGMA: A Focus on <i>BRCA2</i> Variant Classification in Chinese Population.

JCO precision oncology·2026

相关实验视频

Updated: Jul 27, 2025

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.6K

INSurVeyor:改进从短读测序数据中进行插入调用.

Ramesh Rajaby1,2, Dong-Xu Liu3,4, Chun Hang Au1

  • 1Hong Kong Genome Institute, Hong Kong Science Park, Shatin, Hong Kong, China.

Nature communications
|June 5, 2023
PubMed
概括
此摘要是机器生成的。

INSurVeyor显著改善了DNA插入的检测,这是一个常见的结构变异. 这种新方法比现有的工具更灵敏,可以创建更完整的基因组插入目录.

更多相关视频

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.2K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.5K

相关实验视频

Last Updated: Jul 27, 2025

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.6K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.2K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.5K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 插入,定义为对DNA添加50多个核酸的添加,是关键的结构变异.
  • 目前用于检测来自下一代测序 (NGS) 短读的插入的现有方法往往缺乏灵敏度.

研究的目的:

  • 引入INSurVeyor,一种用于从NGS配对端数据中灵敏精确检测插入的新方法.
  • 使用INSurVeyor生成人类和Arabidopsis thaliana基因组的全面插入目录.

主要方法:

  • 开发和应用INSurVeyor,一种用于插入检测的新计算工具.
  • 使用来自人类和非人类生物的基准数据集进行验证.
  • 在1001个基因组项目 (Arabidopsis thaliana) 和1000个基因组项目 (人类) 数据集中编目插入.

主要成果:

  • 与现有的单个和组合呼叫器相比,INSurVeyor表现出更高的灵敏度和精度.
  • 它的性能接近大多数插入类型的长读调用器的性能.
  • 生成的插入目录比当前的资源更完整,更准确,可以识别错过的插入.

结论:

  • INSurVeyor代表了从NGS数据中插入检测的重大进步.
  • 生成的基因组目录增强了我们对人类和植物结构变异的理解.
  • 该方法解决了当前插入检测的局限性,提高了基因组资源的完整性.