通过微细分技术在一根头发中分析了42种精神活性物质
Jiao-Jiao Ji1,2, Duo-Qi Xu1, Ping Xiang1
1Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
Fa yi xue za zhi
|June 5, 2023
概括
这项研究开发了一种敏感的方法,可以在微小的头发段中检测出42种精神活性药物. 这种技术对于调查药物促成的性侵犯案件是有效的.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
背景情况:
- 在生物样本中精确检测药物对于法医调查至关重要.
- 单头发分析为长期药物监测提供了一种方法.
- 开发高灵敏度的方法来分析小毛细节是必不可少的.
研究的目的:
- 建立和验证一种液体染色学-并联质谱法 (LC-MS/MS) 方法.
- 使用单毛微细分技术检测42种精神活性物质.
- 为了分析0.4毫米的头发段,用于药物检测.
主要方法:
- 单个头发样本被细分成0.4毫米的小块.
- 提取方法是使用在含有丁醇的介质中的超声波进行.
- 使用了液体染色学-双重质谱法 (LC-MS/MS) 与正离子模式中的电喷射电离和多重反应监测 (MRM).
主要成果:
- 该方法对42种精神活性物质显示出良好的线性 (r>0.99).
- 检测极限 (LOD) 在0.2-10 pg/mm之间,量化极限 (LOQ) 在0.5-20 pg/mm之间.
- 该方法实现了可接受的精度,准确性,恢复率和矩阵效应,并在人类头发样本中成功检测到了皮.
结论:
- 单毛微细分技术为药物分析提供了一种敏感且可靠的方法.
- 这种经过验证的方法适用于法医应用,特别是在药物促成的性侵犯调查中.
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