微质过程中的局部翻译对于高效的细胞化是必需的
Michael J Vasek1,2, Shayna M Mueller1,2, Stuart B Fass1,2
1Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.
Nature neuroscience
|June 5, 2023
概括
在小鼠大脑中的外周微质过程 (PeMP) 执行局部蛋白质合成. 这种局部翻译对于微质功能如细胞和病原体防御至关重要.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 神经元,星体细胞和寡聚体细胞在它们的远端过程中调节蛋白质翻译.
- 局部翻译在微质过程中的作用,特别是外围微质过程 (PeMPs),仍然在很大程度上未被探索.
研究的目的:
- 研究小鼠大脑中外围微质过程 (PeMPs) 中是否发生受调局部蛋白转化.
- 确定PeMPs中微质活动局部翻译的功能意义.
主要方法:
- 对PeMPs中的核糖体参与和相关转录的分析.
- 现场切片准备以评估翻译阻塞对PeMP功能的影响.
- 观察PeMP行为和细胞因应严重条件和翻译抑制而发生的细胞分裂.
主要成果:
- PeMPs 含有参与 de novo 蛋白质合成的活跃核糖体,与病原体防御,运动性和细胞分裂的转录相关.
- 在PeMPs中抑制急性翻译损害了 fagocytic杯的形成, lysosomal蛋白的局部化,以及apoptotic细胞和病原体样粒子的 fagocytosis.
- 一些PeMP已经证明并要求新的本地蛋白质合成来有效地进行病原体样颗粒的细胞化.
结论:
- 这些发现为外围微质过程中受调节的局部蛋白转化提供了证据.
- 在PeMPs中的局部蛋白质合成对于支持动态的微质功能至关重要,包括细胞形成和对病原体的反应.
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