在海洋甲原体中进行同化硫酸盐的减少 Methanothermococcus thermolithotrophicus
Marion Jespersen1, Tristan Wagner2
1Microbial Metabolism Group, Max Planck Institute for Marine Microbiology, Bremen, Germany.
Nature microbiology
|June 5, 2023
概括
甲热菌 (Methanothermococcus thermolithotrophicus) 独特地使用硫酸盐来促进生长,它结合了甲生成和硫酸盐减少. 它独特的硫酸盐同化途径涉及非典型的酶,可能是从其他微生物中获得和重新使用的.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 考古物 (Archaea) 是一种古老的物种.
背景情况:
- 甲热菌 (Methanothermococcus thermolithotrophicus) 是一种独特的甲原体,使用硫酸盐作为其唯一的硫来源.
- 这种有机体独特地将甲生成与硫酸盐还原途径相结合.
研究的目的:
- 为了阐明在Methanothermococcus thermolithotrophicus中完全的硫酸盐还原路径.
- 为了描述参与硫酸盐同化中的酶,在这种甲原体中.
主要方法:
- 生理学分析 生理学分析
- 生物化学测定 生物化学测定
- 结构分析 结构分析
主要成果:
- 确定了非典型的酶,催化了硫酸盐还原途径的后续步骤.
- 通过PAPS还原酶证明了3'-adenosine 5'-硫酸盐 (PAPS) 的转化为硫酸盐和3'-adenosine 5'-酸盐 (PAP).
- 描述了一种非正规的PAP酸酶和F420依赖的硫酸盐还原酶,用于硫化物生产.
结论:
- 在M. thermolithotrophicus中,硫酸盐同化途径与其他甲基生物有所区别.
- 这种途径可能是由横向基因转移和微生物酶的重新用途造成的.
- 这种独特的代谢能力在微生物生态系统中起着特殊的作用.
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