两种菌体的表征,感染了来自韩国反动物的Streptococcus bovis/equinus复合体 (SBSEC)
Seon Young Park1, Hyemin Kwon2, Sang Guen Kim3
1Division of Animal and Dairy Sciences, College of Agriculture and Life Science, Chungnam National University, Daejeon, 34134, South Korea.
Scientific reports
|June 5, 2023
概括
描述了两种针对Streptococcus bovis/equinus复合体 (SBSEC) 细菌的新型细菌菌体. 这些菌体显示出潜力作为生物控制剂对SBSEC及其生物膜在动物环境中.
科学领域:
- 微生物学和病毒学
- 流体微生物学 流体微生物学
- 菌体的研究研究研究.
背景情况:
- 菌株Streptococcus bovis/equinus复合体 (SBSEC) 是乳汁中乳酸的关键生产者,有助于亚急性乳汁酸症.
- 尽管细菌具有重要意义,但在小肠中准SBSEC的菌体 (菌体) 了解得很少.
- 感染SBSEC的菌体的表征对于了解牛微生物动态和开发控制策略至关重要.
研究的目的:
- 在生物学和基因组学上对两种感染SBSEC物种的新型菌体进行表征,包括Streptococcus ruminicola.
- 评估这些菌体的稳定性和传染性,在模仿乳头环境的条件下.
- 评估这些菌体作为生物控制剂对抗SBSEC细菌及其生物膜的潜力.
主要方法:
- 针对SBSEC的两个菌体 (vB_SbRt-pBovineB21和vB_SbRt-pBovineS21) 的分离和表征.
- 形态分析 (类似Podoviridae),宿主范围的确定 (SBSEC,乳球菌,乳球菌) 和稳定性测试 (热,pH).
- 基因组测序,植物遗传学分析 (Fischettivirus),以及对浮游生物细菌和生物膜的细菌溶解活性的评估.
主要成果:
- 隔离的菌体在形态上与Podoviridae相似,并感染多种SBSEC物种,乳球菌和乳球菌.
- 菌体具有很高的热和pH稳定性,表明它适应食动物的环境,包括低pH条件.
- 基因组分析将菌体置于Fischettivirus属内,与Streptococcus菌体C1不同,有效抑制浮游生物SBSEC生长和预防细菌生物膜.
结论:
- 两种新型的菌体,vB_SbRt-pBovineB21和vB_SbRt-pBovineS21,已经被确定并被描述为Fischettivirus属的新成员.
- 这些菌体表现出强大的稳定性和对SBSEC和其他乳酸产生细菌的广泛活性,包括生物膜抑制.
- 描述的菌体代表了有希望的生物控制策略的候选人,以食SBSEC细菌为目标,并减轻亚急性食酸性疾病.
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