RegiSTORM:用于多色随机光学重建显微镜的通道注册
Øystein Øvrebø1,2,3, Miina Ojansivu4, Kimmo Kartasalo5
1Department of Materials, Imperial College London, London, SW7 2AZ, UK.
BMC bioinformatics
|June 5, 2023
概括
在多色STORM显微镜中,RegiSTORM软件通过使用信托标记来纠正道错位. 这种开源工具可以提高生物相互作用研究的图像准确性.
科学领域:
- 显微镜的使用方法
- 生物物理学的生物物理.
- 计算生物学 计算生物学
背景情况:
- 随机光学重建显微镜 (STORM) 能够实现超分辨率成像,但在长时间采集时会受到样本漂移的影响.
- 在多色STORM中,连续的通道获取导致通道间的错位,阻碍了对生物相互作用的研究.
- 现有的漂移校正方法不能完全解决多色STORM.中的通道间错位问题.
研究的目的:
- 开发和验证一个软件解决方案,RegiSTORM,用于在STORM显微镜中准确地记录通道.
- 为了克服多色STORM成像中的通道间漂移的局限性.
- 用精确的多色STORM数据来增强生物相互作用的表征.
主要方法:
- RegiSTORM使用STORM本地化数据中的信托标记作为准确的频道注册的地标.
- 信托人是根据其在STORM数据中的不眼特征来识别的.
- 该软件在信托记录,细胞样本和脂质纳米粒子上进行了测试.
主要成果:
- 在信托记录中,RegiSTORM在所有测试的道组合中显著减少了目标注册错误.
- 该软件在多染色细胞样本 (管氨酸) 中证明了有效的通道注册.
- 即使没有信托,RegiSTORM也成功地注册了脂质纳米颗粒的双色STORM记录,显示了广泛的适用性.
结论:
- RegiSTORM准确地记录了多个STORM通道,有效地减轻了通道间错位.
- 该软件作为开源软件免费提供,促进其在研究中的采用.
- 在复杂的生物研究中,RegiSTORM提高了多色STORM的可靠性.
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